QTL mapping of volatile compound production in Saccharomyces cerevisiae during alcoholic fermentation

BMC Genomics
Matthias EderSylvie Dequin

Abstract

The volatile metabolites produced by Saccharomyces cerevisiae during alcoholic fermentation, which are mainly esters, higher alcohols and organic acids, play a vital role in the quality and perception of fermented beverages, such as wine. Although the metabolic pathways and genes behind yeast fermentative aroma formation are well described, little is known about the genetic mechanisms underlying variations between strains in the production of these aroma compounds. To increase our knowledge about the links between genetic variation and volatile production, we performed quantitative trait locus (QTL) mapping using 130 F2-meiotic segregants from two S. cerevisiae wine strains. The segregants were individually genotyped by next-generation sequencing and separately phenotyped during wine fermentation. Using different QTL mapping strategies, we were able to identify 65 QTLs in the genome, including 55 that influence the formation of 30 volatile secondary metabolites, 14 with an effect on sugar consumption and central carbon metabolite production, and 7 influencing fermentation parameters. For ethyl lactate, ethyl octanoate and propanol formation, we discovered 2 interacting QTLs each. Within 9 of the detected regions, we validated t...Continue Reading

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Citations

Nov 18, 2018·FEMS Microbiology Reviews·Sylvester HoltJohan M Thevelein
Feb 26, 2020·Microbial Biotechnology·Wladimir MardonesFrancisco A Cubillos
Apr 26, 2019·Applied Microbiology and Biotechnology·Antonio G CordenteChristopher D Curtin
Nov 15, 2019·Journal of Agricultural and Food Chemistry·Niël van WykIsak S Pretorius
Nov 27, 2020·Frontiers in Genetics·Jennifer Molinet, Francisco A Cubillos
Jun 10, 2021·Applied Microbiology and Biotechnology·Louise BartlePhilippe Marullo
Jun 27, 2021·Microbial Biotechnology·Ramon Gonzalez, Pilar Morales
Dec 18, 2021·Biotechnology for Biofuels·Alessandro L V CoradiniGleidson Silva Teixeira

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Methods Mentioned

BETA
PCA
genotyping
PCR

Software Mentioned

FASTX Toolkit
RHA
YEASTRACT
TRIMMOMATIC
BWA
R
eqtl
qtl
SAMtools

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