May 1, 2016

QTL mapping reveals complex genetic architecture of quantitative virulence in the wheat pathogen Zymoseptoria tritici.

BioRxiv : the Preprint Server for Biology
E L StewartBruce A McDonald

Abstract

We conducted a comprehensive analysis of virulence in the fungal wheat pathogen Zymoseptoria tritici using QTL mapping. High throughput phenotyping based on automated image analysis allowed measurement of pathogen virulence on a scale and with a precision that was not previously possible. Across two mapping populations encompassing more than 520 progeny, 540,710 pycnidia were counted and their sizes and grey values were measured, yielding over 1.6 million phenotypes associated with pathogen reproduction. Large pycnidia were shown to produce more numerous and larger spores than small pycnidia. Precise measures of percent leaf area covered by lesions provided a quantitative measure of host damage. Combining these large and accurate phenotype datasets with a dense panel of RADseq genetic markers enabled us to genetically dissect pathogen virulence into components related to host damage and components related to pathogen reproduction. We show that different components of virulence can be under separate genetic control. Large- and small-effect QTLs were identified for all traits, with some QTLs specific to mapping populations, cultivars and traits and other QTLs shared among traits within the same mapping population. We associated t...Continue Reading

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Mentioned in this Paper

Biological Markers
Rathayibacter tritici
Quantitative Trait Loci
Size
Cell Injury
Pathogenic Organism
Reproduction
Candidate Disease Gene
Zymoseptoria tritici
Reproduction Spores

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