Quadruplex-Templated and Catalyzed Ligation of Nucleic Acids.

Chembiochem : a European Journal of Chemical Biology
Besik Kankia

Abstract

Template-guided chemical reactions between nucleic acid strands are an important process in biomedical research. However, almost all of these reactions employ an oligonucleotide-templated approach that is based on the double-helix alignment. The moderate stability of the double helix makes this approach unsuitable for many chemical reactions, so alternative nucleic acid alignment mechanisms, demonstrating higher thermal and chemical stability, are desirable. Earlier, we described a noncovalent coupling mechanism between DNA strands through a quadruplex-and-Mg2+ connection (QMC). QMC is based on G-quadruplexes and allows unusually stable and specific interactions. Herein, a novel catalytic nucleic acid reaction, based on QMC, is described. This approach uses G-tetrads as a structural and recognition element without employing Watson-Crick complementarity rules at any stage of substrate/catalyst formation or interaction between them. Quadruplex-templated ligation can be achieved through the self-ligation of two nucleic acid strands, or through a quadruplex catalyst, which forms a G-triplex and specifically connects the strands. The process is extraordinarily robust and efficient. For instance, the ligation of carbodiimide-activate...Continue Reading

References

Feb 25, 1995·Nucleic Acids Research·T C MarshE Henderson
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Apr 3, 2007·FEBS Letters·Phillip A RachwalKeith R Fox
Mar 26, 2011·Biophysical Chemistry·Sean KelleyBesik I Kankia
Oct 19, 2012·Organic & Biomolecular Chemistry·Claudia PercivalleSylvain Ladame
Jan 22, 2013·Angewandte Chemie·Vittorio LimongelliMichele Parrinello
Dec 10, 2013·Biophysical Chemistry·Jordan MathiasBesik Kankia
May 20, 2014·The Journal of Physical Chemistry. B·Besik Kankia
Nov 8, 2014·Nucleic Acids Research·Linda CerofoliniClaudio Luchinat
Aug 13, 2015·Scientific Reports·Besik Kankia
May 26, 2016·Biophysical Journal·Besik KankiaKarin Musier-Forsyth
Jul 16, 2017·Chemistry & Biodiversity·Markus Kramer, Clemens Richert
Mar 7, 2018·Nature Chemistry·Zhen ChenDavid R Liu
Mar 14, 2018·Chemistry : a European Journal·Brooke A Anderson, Ramanarayanan Krishnamurthy
Apr 9, 2020·Chembiochem : a European Journal of Chemical Biology·Valerie Rauser, Elmar Weinhold
Jul 25, 2020·Chembiochem : a European Journal of Chemical Biology·Chiamaka ObianyorNicholas V Hud

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Citations

Jul 3, 2021·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Besik Kankia

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