Quadruplex Turncoats: Cation-Dependent Folding and Stability of Quadruplex-DNA Double Switches

Journal of the American Chemical Society
Eric LargyJean-Louis Mergny

Abstract

Quadruplex (G4) nucleic acids, a family of secondary structures formed by guanine-rich sequences, exhibit an important structural polymorphism. We demonstrate here that G-rich DNA sequences may function as a double switch based on different triggers, provided that their quadruplex structures and stability display a high dependence on cation nature and concentration. A first switch is based on a remarkable antiparallel-to-parallel conversion, taking place in a few seconds at room temperature by addition of low KCl amounts to a sodium-rich sample. The second switch involves the conversion of alternative antiparallel quadruplex structures binding only one cation, formed in the presence of sub-millimolar potassium or strontium concentrations, to parallel structures by increasing the cation concentration. Incidentally, extremely low K(+) or Sr(2+) concentrations (≤5 equiv) are sufficient to induce G4 formation in a buffer devoid of other G4-promoting cations, and we suggest that the alternative structures observed contain only two tetrads. Such DNA systems are biological relevant targets, can be used in nanotechnology applications, and are valuable methodological tools for understanding DNA quadruplex folding, notably at low cation ...Continue Reading

References

Feb 4, 1994·Journal of Molecular Biology·P SchultzeJ Feigon
May 20, 1999·Biopolymers·V K Misra, D E Draper
Nov 1, 2001·Journal of the American Chemical Society·B I Kankia, L A Marky
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J DengM Sundaralingam
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
May 9, 2003·Nucleic Acids Research·Eric EnnifarPhilippe Dumas
Mar 18, 2004·Oligonucleotides·Jean-Louis Mergny, Laurent Lacroix
Jun 25, 2004·Journal of Biomolecular Structure & Dynamics·X MaoW H Gmeiner
Jun 30, 2005·Nucleic Acids Research·Primoz SketJanez Plavec
Sep 15, 2005·Nucleic Acids Research·Jean-Louis MergnyJonathan B Chaires
Oct 3, 2006·Nucleic Acids Research·Sarah BurgeStephen Neidle
Oct 13, 2006·Organic & Biomolecular Chemistry·Patrizia AlbertiJean-Louis Mergny
May 9, 2007·Biochemical and Biophysical Research Communications·Ilene M PedrosoTerace M Fletcher
Nov 1, 2007·Chemistry : a European Journal·Mateus Webba da Silva
Apr 1, 2008·Biochimie·Jixun DaiDanzhou Yang
Apr 29, 2008·Nucleic Acids Research·Mark JohnsonThomas L Madden
Jun 17, 2008·The Journal of Physical Chemistry. B·Amit Arora, Souvik Maiti
Jul 17, 2008·Journal of the American Chemical Society·Nicolas SmargiassoValérie Gabelica
Dec 24, 2008·Nucleic Acids Research·Samir AmraneAnh Tuân Phan
Jul 8, 2009·Nucleic Acids Research·Aurore GuédinJean-Louis Mergny
Aug 27, 2009·The Journal of Physical Chemistry. B·Robert D GrayJonathan B Chaires
Jul 19, 2011·Biophysical Chemistry·Robert D Gray, Jonathan B Chaires
Aug 4, 2011·Angewandte Chemie·Florian HamonMarie-Paule Teulade-Fichou
Sep 9, 2011·Chemical Society Reviews·Jungkweon Choi, Tetsuro Majima
Sep 20, 2011·Angewandte Chemie·Andreas Ioannis KarsisiotisMateus Webba da Silva
Sep 23, 2011·Organic & Biomolecular Chemistry·M Clarke MillerJohn O Trent
Oct 25, 2011·Chemical Communications : Chem Comm·Mariana Martín-Hidalgo, José M Rivera
Mar 1, 2012·Chemistry : a European Journal·Aurore De RacheClaudine Buess-Herman
Mar 2, 2012·Current Pharmaceutical Design·Eric LargyMarie-Paule Teulade-Fichou
Apr 25, 2012·Methods : a Companion to Methods in Enzymology·Robert D Gray, Jonathan B Chaires
Sep 20, 2012·Journal of the American Chemical Society·Robert D GrayJonathan B Chaires

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Citations

Jun 21, 2016·The Journal of Physical Chemistry. B·Dongju ZhangXiaohong Li
Dec 8, 2016·Nucleic Acids Research·Adrien Marchand, Valérie Gabelica
Feb 15, 2017·The Journal of Physical Chemistry. B·Adam K SieradzanDavid J Wales
May 20, 2017·Journal of the American Chemical Society·Jun ZhouJean-Louis Mergny
Mar 30, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Xiaochun HuShuo Shi
Jun 7, 2016·Chemical Communications : Chem Comm·Lei HeFangwei Shao
May 18, 2016·Physical Chemistry Chemical Physics : PCCP·F ZaccariaC Fonseca Guerra
Jul 22, 2019·FEBS Letters·Helen L LightfootJonathan Hall
Jun 3, 2020·Chemical Communications : Chem Comm·Heng GaoYong Shao
Sep 4, 2019·Journal of Biomolecular Structure & Dynamics·Ji Hoon HanYoon Jung Jang
Apr 22, 2020·Nucleic Acids Research·Michela ZuffoAnton Granzhan
Dec 5, 2016·Nucleic Acids Research·Artemy D BeniaminovDmitry N Kaluzhny
Aug 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Filippo DoriaMauro Freccero
Jun 4, 2020·International Journal of Molecular Sciences·Claudia RiccardiDaniela Montesarchio
Aug 11, 2020·Pharmacology & Therapeutics·Claudia RiccardiDaniela Montesarchio
Oct 19, 2017·Journal of the American Society for Mass Spectrometry·Steven DalyFabien Chirot
Jan 4, 2019·Chemical Reviews·Jean-Louis Mergny, Dipankar Sen
Dec 30, 2017·Journal of the American Chemical Society·Hisae Tateishi-KarimataNaoki Sugimoto
Nov 13, 2020·The Journal of Physical Chemistry Letters·Rajesh Kumar Reddy SannapureddiBharathwaj Sathyamoorthy
Aug 23, 2019·Journal of the American Chemical Society·Prashant S DeoreRichard A Manderville
Feb 11, 2020·Analytical Chemistry·Eric Largy, Valérie Gabelica

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