Qualifying label components for effective biosensing using advanced high-throughput SEIRA methodology

Physical Chemistry Chemical Physics : PCCP
Andrea HornemannBurkhard Beckhoff

Abstract

The need for technological progress in bio-diagnostic assays of high complexity requires both fundamental research and constructing efforts on nano-scaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. Nanoparticle induced sensitivity enhancement and its application related to multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are well suitable for these purposes. The potential of diverse fluorophore-antibody conjugates, being chemisorbed onto low-cost gold nanoparticulate SEIRA substrates, has been explored with respect to their spectral discriminability. These novel biolabels deliver molecular SEIRA fingerprints that have been successfully analyzed by both uni- and multivariate analyzing tools, to discriminate their multiplexing capabilities. We show that this robust spectral encoding via SEIRA fingerprints opens up new opportunities for a fast, reliable and multiplexed high-end screening in biodiagnostics.

References

Nov 4, 1970·Journal of the American Chemical Society·A W Herlinger, T V Long
Aug 17, 2001·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·L WangA K Gaigalas
Mar 15, 2003·Bioscience Reports·Alexander A KamnevMoschos G Polissiou
Jul 30, 1960·Nature·F S PARKER, D M KIRSCHENBAUM
Jan 13, 1962·Nature·H RINDERKNECHT
Dec 5, 2006·International Journal of Pharmaceutics·Fei TianJ Howard Rytting
Oct 5, 2007·Journal of Environmental Sciences (China)·Yan-fen FangDavid Johnson
Sep 25, 2009·Biosensors & Bioelectronics·Anjum QuershiJimmy L Davidson
Oct 2, 2009·Analytical and Bioanalytical Chemistry·Mehmet KahramanMustafa Culha
Oct 4, 2011·Nature Biotechnology·Peng QiuSylvia K Plevritis

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