Qualitation and quantification of water soluble non-starch polysaccharides from Pseudostellaria heterophylla in China using saccharide mapping and multiple chromatographic methods

Carbohydrate Polymers
Yong DengShao-Ping Li

Abstract

Qualitative and quantitative analysis of non-starch polysaccharide (NSP) from Pseudostellaria heterophylla in China (Guizhou, Anhui and Fujian) were performed using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), saccharide mapping based on polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) and high performance thin layer chromatography (HPTLC). Results showed that PACE and HPTLC fingerprints of enzymatic hydrolysates of NSP were similar. Moreover, the results indicated that 1,4-β-d-Galp, 1,5-α-Araf, 1,4-α-d-GalAp and 1,4-β-d-Glcp linkages existed in NSP. Based on the results of HPSEC-MALLS/RI, the Mw, Rg, and contents of NSP in P. heterophylla were ranging from 4.37 × 104 to 1.70 × 105 Da, 46.4 to 65.9 nm, and 0.43% to 1.41%, respectively. There were no significant difference (p>0.05) observed among the three main producing areas, which suggested that polysaccharides could be used as marker for quality control of P. heterophylla.

Citations

Dec 18, 2020·Natural Product Research·Xiao-Fei ZhaoQian-Feng Chen
Nov 18, 2018·International Journal of Biological Macromolecules·Huan LiWei Liu

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