Qualitative and quantitative analysis of amino acids by capillary electrophoresis-electrospray ionization-tandem mass spectrometry

Electrophoresis
Tomoyoshi SogaTakaaki Nishioka

Abstract

We describe a method to identify and quantify amino acids using capillary electrophoresis-electrospray ionization-triple-quadrupole tandem mass spectrometry (CE-ESI-MS/MS). Amino acids, including physiological amino acids, were first separated by CE under acidic pH conditions and then detected by MS/MS. To efficiently introduce the whole sample into the capillary, no electrical potential was applied to the electrospray probe until running electrophoresis. The position of the electrosprayer with respect to the MS capillary entrance drastically affected sensitivity and generation of cluster ions. MS/MS with multiple reaction monitoring (MRM) detection was performed to obtain sufficient selectivity and sensitivity. Under optimized CE-MS/MS conditions, the minimum detectable levels for 32 free amino acids normally found in proteins and other physiological amino acids were between 0.1 and 14 micromol/L with pressure injection of 50 mbar for 3 s (3 nL) at a signal-to-noise ratio of 3. For most amino acids, this constitutes a severalfold increase in sensitivity compared to CE-MS. The relative standard deviations (% RSD) for all amino acids were better than 0.4% for migration times and between 1.4% and 8.6% for peak areas (n = 10). Sin...Continue Reading

Citations

May 29, 2008·Analytical and Bioanalytical Chemistry·Björn ThieleDiana Hofmann
Oct 10, 2008·Analytical and Bioanalytical Chemistry·Hannelore KasparPeter J Oefner
Apr 27, 2005·The Analyst·Warwick B DunnHelen E Johnson
Oct 6, 2005·Theoretical Biology & Medical Modelling·Katsuyuki YugiMasaru Tomita
Nov 6, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Eddy Wing Yin NgTerence Chuen Wai Poon
Jan 27, 2005·Journal of Mass Spectrometry : JMS
Jan 15, 2013·Chemico-biological Interactions·Zhengzhi XieOleg A Barski
Mar 12, 2005·Electrophoresis·Carolina SimóAlejandro Cifuentes
Jun 21, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rawi RamautarGerhardus J de Jong
Mar 15, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Naz ShamaByung Hwa Jung
Feb 5, 2008·Journal of Chromatography. a·Petr HusekHelena Zahradnícková
Aug 21, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Birgitte WuytsLinde Goossens
Jun 2, 2007·Journal of Chromatography. a·Oleg A MayborodaAndre M Deelder
Mar 23, 2007·Journal of Chromatography. a·Maria Rowena N Monton, Tomoyoshi Soga
Oct 28, 2005·Electrophoresis·Jens OhnesorgeHermann Wätzig
Mar 19, 2005·Electrophoresis·Philippe Schmitt-Kopplin, Matthias Englmann
Jun 12, 2009·Electrophoresis·Christian W Klampfl
Dec 23, 2006·Journal of Mass Spectrometry : JMS·Anne Marie Scharff-PoulsenHelge Egsgaard
Jan 22, 2009·Electrophoresis·Pavla PantůckováLudmila Krivánková
Jan 20, 2006·Electrophoresis·Véréna PoinsotFrançois Couderc
Apr 12, 2007·Electrophoresis·Maike SniehottaHarald Mischak
Dec 25, 2008·Electrophoresis·Rawi RamautarGerhardus J de Jong
Mar 30, 2007·Journal of Separation Science·Rania BakryGünther K Bonn
Nov 30, 2005·Electrophoresis·Christian W HuckGünther K Bonn
Sep 11, 2012·Journal of Separation Science·Tom AthertonAna M García-Campaña

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