Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography

Journal of Pharmaceutical and Biomedical Analysis
J S ZhangS P Li

Abstract

The rhizomes of Curcuma phaeocaulis, Curcuma kwangsiensis, Curcuma wenyujin and Curcuma longa are used as Ezhu or Jianghuang in traditional Chinese medicine for a long time. Due to their similar morphological characters, it is difficult to distinguish their origins of raw materials used in clinic. In this study, a simple, rapid and reliable twice development TLC method was developed for qualitative and quantitative analysis of the four species of Curcuma rhizomes. The chromatography was performed on silica gel 60F(254) plate with chloroform-methanol-formic acid (80:4:0.8, v/v/v) and petroleum ether-ethyl acetate (90:10, v/v) as mobile phase for twice development. The TLC markers were colorized with 1% vanillin-H(2)SO(4) solution. The four species of Curcuma were easily discriminated based on their characteristic TLC profiles, and simultaneous quantification of eight compounds, including bisdemethoxycurcumin, demethoxycurcumin, curcumine, curcumenol, curcumol, curdione, furanodienone and curzerene, in Curcuma were also performed densitometrically at lambda(scan)=518nm and lambda(reference)=800 nm. The investigated compounds had good linearity (r(2)>0.9905) within test ranges. Therefore, the developed TLC method can be used for q...Continue Reading

References

Feb 16, 2006·Rapid Communications in Mass Spectrometry : RCM·Hongliang JiangDavid R Gang

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Citations

Dec 18, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Jing ZhangJian-Bo Wan
Apr 9, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Yang ZhouGaimei She
Dec 19, 2012·Journal of Pharmaceutical and Biomedical Analysis·Changhua XuSuqin Sun
Oct 13, 2009·European Journal of Pharmacology·Supachai YodkeereePornngarm Limtrakul
Dec 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yingqian CiMei Han
Oct 30, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing ZhaoShao-Ping Li
Apr 15, 2020·Nutrition and Cancer·Sheema HashemAjaz A Bhat

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