Qualitative and quantitative determination of poloxamer surfactants by mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Z TakátsL Hegedüs

Abstract

Poloxamers are polyethylene-polypropylene glycol linear co-polymers. A simple matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method has been developed for the determination of the average molecular weight of poloxamers. The molecular mass of five standard poloxamers determined by MALDI closely corresponds to that specified by the manufacturers, and no mass distribution effects were observed. Quantitation of distributions based on the molecular mass envelope using electrospray (ES) ionization was unsuccessful. To overcome this problem, quantitation was based on fragment ions (m/z 45 and 59) which gave reproducible signals using a very high orifice voltage ( approximately 200 eV). Poloxamer concentrations were determined accurately with a good linear response using the standard addition method. We believe that the use of very small fragment ions for quantitation of polymers may become a widely applicable general technique.

References

Mar 1, 1984·Journal of Pharmaceutical Sciences·F Guay, S Bisaillon
Apr 17, 1998·Atherosclerosis·W K PalmerT P Johnston
May 20, 1998·The Journal of Surgical Research·F A MerchantM Toner
Apr 15, 1999·International Journal of Radiation Biology·J HannigR C Lee
May 20, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·P D ScholesM C Davies
Dec 3, 1999·Antimicrobial Agents and Chemotherapy·C JagannathR L Hunter
Aug 10, 2000·Radiation Research·J HannigR C Lee

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Citations

Nov 11, 2006·Pharmaceutical Research·Gilles DumortierJean Claude Chaumeil
Jan 10, 2008·Journal of Pharmaceutical and Biomedical Analysis·Z Urbán-MorlánD Quintanar-Guerrero
Nov 23, 2006·Neuromuscular Disorders : NMD·John G QuinlanMartin Emanuele
Sep 1, 2004·Journal of Pharmaceutical and Biomedical Analysis·Yun MaoEric W Tsai
Aug 27, 2016·Drug Delivery and Translational Research·Arunprasad Sivaraman, Ajay K Banga
Oct 13, 2001·Journal of Mass Spectrometry : JMS

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