Qualitative and quantitative determination of complicated herbal components by liquid chromatography hybrid ion trap time-of-flight mass spectrometry and a relative exposure approach to herbal pharmacokinetics independent of standards

Journal of Chromatography. a
Yan LiangGuangji Wang

Abstract

To date, the pharmacokinetic research of herbal medicines (HMs) is still in its infancy and is facing critical technical challenges on the qualitative and quantitative analysis of complicated components from biological matrices. Additionally, the lack of authentic standards constitutes another bottleneck on assessing herbal pharmacokinetics. This present work contributes to the development of a powerful technical platform for both qualitative and quantitative pharmacokinetic analysis of herbal components, and a strategy of relative exposure that provides a practicable pharmacokinetic assessment independent of authentic standards, based on the use of liquid chromatography hybrid ion trap time-of-flight mass spectrometry (LC-IT-TOF/MS). Taking schisandra lignans extract (SLE) as an example, the LC-IT-TOF/MS assay was initially applied to the global qualitative analysis of components contained in SLE per se and in the rat plasma post SLE dosing. Afterwards, this study focused on validating the quantitative performance of LC-IT-TOF/MS assay by comparison with a well-established LC-Q/MS assay. For the absolute quantification of five lignans components with authentic standards, both assays showed very similar analytical figures of me...Continue Reading

Citations

Aug 15, 2012·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Alan Hb WuKara L Lynch
Jun 5, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yan LiangGuang-ji Wang
May 7, 2013·Journal of Pharmaceutical and Biomedical Analysis·Fangyuan GaoGuorong Fan
Aug 14, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zhimin LuoQiang Fu

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