Qualitative and quantitative differences in the subgingival microbiome of the restored and unrestored teeth

Journal of Periodontal Research
Steven W H RademacherMarja L Laine

Abstract

Metal-based dental restorations with a subgingival outline may enhance plaque accumulation and bacterial colonization. This study aimed to investigate whether metal-based restorations influence the composition of subgingival microbiome. Per subject one site with a metal-based restoration and one contra-lateral site without a restoration were selected on basis of radiographic bone loss ≤2 mm, restoration outline at sulcus level/subgingivally, pocket depth ≤4 mm, and no root canal treatments. Subgingival samples were collected with sterile paper-points, and microbial profiles were obtained by 16S rRNA gene amplicon sequencing. Restorations were sampled with an Arkansas-stone and the metal composition was determined using energy-dispersive X-ray spectroscopy. A total of 22 sites from 11 subjects were included. No significant differences for the clinical parameters were found between the restored and unrestored sites. The average age of the restorations was 14.9 ± 7.1 years. Firmicutes was the most prevalent phylum at the restored sites (32% vs 20% of the reads of the unrestored sites, P = 0.016), and Actinobacteria at the unrestored sites (33% vs 18% of the reads of the restored sites, P = 0.01). Overall, sequences clustered into ...Continue Reading

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Citations

Mar 4, 2021·Critical Reviews in Microbiology·Lean Heong FooChaminda Jayampath Seneviratne
Feb 21, 2021·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Runzhi GuoWeiran Li
Jun 9, 2021·Journal of Clinical Periodontology·Angeliki PolymeriBernd W Brandt

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Methods Mentioned

BETA
PCR

Software Mentioned

Quantitative Insights in Microbial Ecology ( QIIME
Paleontological
SPSS PASW Statistics
PERMANOVA

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