Qualitative-(semi)quantitative data acquisition of artemisinin and its metabolites in rat plasma using an LTQ/Orbitrap mass spectrometer

Journal of Mass Spectrometry : JMS
Fuying DuJ Xing

Abstract

Artemisinin (QHS) is one of the first-line antimalarials, and autoinduction of CYP-mediated metabolism can result in its reduced exposure. To better understand the autoinduction of QHS, we evaluated the pharmacokinetics of QHS and its phase I metabolites in rats using an liquid chromatography-high resolution mass spectrometry (LC-HRMS) method. The LC separation was improved, allowing the separation of QHS and its metabolites from their diastereomers, and seven metabolites of QHS with relatively high exposure were identified in rat plasma, including deoxyartemisinin (DQHS), three monoyhydroxylated plus deoxyl metabolites (M1-M3) and three monohydroxylated metabolites (M4-M6). For detection, a high-resolution LTQ/Orbitrap mass spectrometer with an electrospray ionization (ESI) inlet in the positive ion mode was used. High-resolution extracted ion chromatograms for each analyte were obtained by processing the full-scan MS dataset with 10 ppm mass tolerance. The plasma samples were pretreated by protein precipitation with acetonitrile. The standard curve was linear (r(2) > 0.99) over the QHS and DQHS concentration range of 5.0-200.0 ng/ml in 50 µl of plasma, which offered sufficient sensitivity and accuracy for the determination of...Continue Reading

References

Dec 3, 1999·British Journal of Clinical Pharmacology·U S Svensson, M Ashton
Apr 5, 2001·Trends in Parasitology·P L OlliaroY Yuthavong
Apr 16, 2004·The Journal of Clinical Investigation·Nicholas J White
Apr 18, 2008·Journal of the American Society for Mass Spectrometry·Alexander ScherlDavid R Goodlett
Jan 24, 2009·Journal of Pharmaceutical and Biomedical Analysis·N LindegardhN P J Day
Mar 6, 2009·Rapid Communications in Mass Spectrometry : RCM·Nanyan Rena ZhangWilliam Bart Emary
Apr 3, 2009·Journal of the American Society for Mass Spectrometry·Kevin P BatemanLester Taylor
Jun 25, 2010·The Analyst·C JunotEric Ezan
Jun 3, 2011·The Journal of Biological Chemistry·Mingshe ZhuW Griffith Humphreys

❮ Previous
Next ❯

Citations

Jun 13, 2013·Environmental Science & Technology·Junho JeonJuliane Hollender
Apr 17, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Raimo A Ketola, Timo Mauriala
Jan 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yue MaLan Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.