PMID: 9440821Jan 24, 1998Paper

Quality assurance of stem cell enumeration by flow cytometry. Canadian QASE Study Group

Cytometry
I Chin-YeeD R Sutherland

Abstract

The enumeration of CD34+ cells by flow cytometry is commonly employed to assess progenitor/stem cell numbers in peripheral blood, cord blood, and apheresis products used for peripheral blood stem cell transplantation (PBSCT). Until recently, the enumeration of CD34+ cells has evolved in the absence of any procedural guidelines or quality assurance programs. We established a voluntary quality assurance for stem cell enumeration (QASE) program for Canadian laboratories involved in PBSCT. The goals of this study were 1) to survey current flow cytometric procedures for CD34 enumeration; 2) to evaluate inter-institutional reproducibility of currently employed assays; and 3) to evaluate the impact of adoption of a common method on inter-laboratory variability. Fourteen to nineteen laboratories participated in this program by enumerating CD34+ cells in shared cord blood samples. The survey showed a wide variation in flow cytometric methodology which in part contributed to the wide range in results obtained between different centres. Major differences in methodology included the number of events counted, use of isotype control and gating strategy. Participation in this program and adoption of a common methodology increased level of agr...Continue Reading

Citations

Jul 31, 2012·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·D LysákV Koza
May 27, 2003·Journal of Medical Virology·Hiroki IsomuraMasao Yamada
Apr 3, 2007·Cytometry. Part B, Clinical Cytometry·Wilfried H B M LeveringJan W Gratama
Dec 21, 2006·Cytometry. Part B, Clinical Cytometry·Bruno BrandoJan Willem Gratama
Aug 22, 2017·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Rahul NaithaniGaurav Dixit
Jul 8, 2011·Archives of Pathology & Laboratory Medicine·Alain NgomaKenneth E Nollet

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