Quality assurance trials for Ki67 assessment in pathology

Virchows Archiv : an International Journal of Pathology
M RaapHans Kreipe

Abstract

Ki67 is a broadly used proliferation marker in surgical pathology with an obvious need for standardization to improve reproducibility of assessment. Here, we present results of the so far only existing round robin tests on Ki67, organized annually in Germany, Austria, and Switzerland from 2010 to 2015 with up to 160 participating laboratories (QuIP). In each quality assessment trial, eight probes from each breast cancer, neuroendocrine tumor, and malignant lymphoma were compiled on a tissue microarray (TMA). TMAs were stained in the participants' laboratories with antibodies and procedures also applied in their daily routine. Participating pathologists were expected to assign Ki67 values to one of four different categories for each tumor type. All local stainings and evaluations were reassessed by the organizing panel and compared to a preset standard. On average, 95% of participants reached the benchmark of over 80% concordance rates with the Ki67 category pre-established by the panel. Automatization and type of antibody did not affect the success rate. Concordance rates differed between tumor entities being highest in each tumor type with either very high or very low labeling indices. Lower rates were seen for intermediate Ki...Continue Reading

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Citations

Sep 10, 2020·Frontiers in Oncology·Gervásio Teles Cardoso de CarvalhoRenata Toscano Simões
Apr 13, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Solène-Florence Kammerer-JacquetDaniel M Berney
Jan 25, 2020·Virchows Archiv : an International Journal of Pathology·Giorgio A CrociWolfram Klapper
Dec 29, 2020·Journal of the National Cancer Institute·Torsten O NielsenDaniel F Hayes
May 19, 2021·Journal of the National Cancer Institute·Torsten O NielsenUNKNOWN International Ki67 in Breast Cancer Working Group

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