Quality control and single nucleotide resolution analysis of methylated DNA immunoprecipitation products

Analytical Biochemistry
Jennifer SengenèsJörg Tost

Abstract

DNA methylation patterns are altered in many diseases, and their analysis has become of great interest. Methylated DNA immunoprecipitation (MeDIP) is a simple method to enrich the methylated fraction of the genome. However, it has been difficult to assess the quality and the detailed methylation patterns of the immunoprecipitated DNA. Here we present a simple method for the analysis of the immunoprecipitated DNA at single nucleotide resolution by bisulfite treatment and pyrosequencing of genomic regions. The presented method can be used as an initial quality measure prior to genome-wide read-out technologies such as microarrays and second-generation sequencing.

References

Sep 8, 2004·Analytical Biochemistry·Jean-Michel DupontIvo Glynne Gut
Feb 18, 2006·Nature Reviews. Drug Discovery·Christine B Yoo, Peter A Jones
Feb 27, 2007·The American Journal of Pathology·Rossa W K ChiuY M Dennis Lo
Sep 15, 2007·Nature Protocols·Jörg Tost, Ivo G Gut
Sep 16, 2009·Carcinogenesis·Shikhar SharmaPeter A Jones
Feb 4, 2010·Nature Reviews. Genetics·Peter W Laird

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Citations

Oct 31, 2015·New Biotechnology·Steven McGinnIvo G Gut

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