Quality control in diagnostic molecular pathology in the Netherlands; proficiency testing for patient identification in tissue samples

Journal of Clinical Pathology
F B J M ThunnissenDutch Pathology Molecular Diagnostic Working Groups

Abstract

To describe the evolution of proficiency testing for molecular diagnostic pathology with respect to determining unambiguously the patient identity of tissue samples by microsatellite analysis. Four rounds of quality control exchanges of samples from different patients were sent with the purpose of identifying the correct origin of these samples. The samples were either paraffin wax embedded sections on glass, sections in tubes, or isolated DNA. Blinded samples were distributed to all participating laboratories. No restrictions to the method and short tandem repeat markers used for identification were imposed. In four subsequent rounds the number of participating laboratories increased from three to 10. The numbers of samples tested increased in time from five to 12. The microsatellite markers used by the different laboratories showed little overlap. In the first three rounds, in which isolated DNA was provided, all samples were accurately classified irrespective of the microsatellite markers used. In the last round, which also included paraffin wax embedded sections, a small number of laboratories experienced problems, either with amplification or incorrect classification of a few samples. Proficiency testing was useful, and sh...Continue Reading

References

Jan 1, 1997·Cytometry·F B ThunnissenU Jutting
Aug 30, 2000·Virchows Archiv : an International Journal of Pathology·A D CabrasH Höfler
Feb 28, 2001·The Journal of Molecular Diagnostics : JMD·M J WorshamR J Zarbo
Sep 8, 2001·European Journal of Pediatrics·C R Müller, UNKNOWN European Molecular Genetics Quality Network

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Citations

Apr 25, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J H van KriekenUNKNOWN Quality Assurance for Molecular Pathology group
Dec 20, 2012·Virchows Archiv : an International Journal of Pathology·J Han van KriekenElisabeth Dequeker
Mar 2, 2016·Archives of Pathology & Laboratory Medicine·Erik ThunnissenDouglas B Flieder
Jan 23, 2015·Archives of Pathology & Laboratory Medicine·Jonathan DudleyMing-Tseh Lin
Mar 2, 2018·Archives of Pathology & Laboratory Medicine·Matteo Fassan

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