PMID: 8947824Oct 1, 1996Paper

Quality control in IVF with mouse bioassays: a four years' experience

Journal of Assisted Reproduction and Genetics
M van den BerghY Englert

Abstract

We analyzed retrospectively the relevance of 4 years of quality control on homemade culture medium with the mouse IVF and zygote bioassay. In vitro or in vivo fertilized mouse oocytes were cultured in each batch of medium. Two-cell-stage and expanded blastocyst development was recorded for each batch of medium. Data on fertilization and embryo quality obtained in human in vitro fertilization were recorded for each batch. IVF treatment cycles for male infertility and cycles with sperm microinjection were excluded. Human oocyte fertilization dropped from 60 to 54%, respectively, from 57 to 41% in a significant way (P < 0.05 resp. P < 0.01) and the human mean embryo score decreased from 4.17 +/- 1.21 to 3.69 +/- 1.06 (P < 0.05) when media were used with a low two-cell-stage development (< or = 75%) for the mouse zygote or mouse IVF bioassay. The pregnancy rate was not affected. Media with high scores in mouse bioassays show higher fertilization rates and better embryo quality when used for human IVF.

References

Aug 1, 1991·Journal of in Vitro Fertilization and Embryo Transfer : IVF·D E Johnson, G D Hodgen
Feb 1, 1991·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M L Reed, R M Petters
Apr 1, 1989·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M G DodsonJ L Robertson
Apr 1, 1989·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M A George, B G Doe
Jun 1, 1988·Journal of in Vitro Fertilization and Embryo Transfer : IVF·A DavidsonR J Paulson
Dec 1, 1988·Journal of in Vitro Fertilization and Embryo Transfer : IVF·J S RinehartM Gerrity
Jan 1, 1988·Fertility and Sterility·S FishelB Faratian
Apr 1, 1988·Journal of in Vitro Fertilization and Embryo Transfer : IVF·B D Bavister, J C Andrews
Nov 1, 1987·Human Reproduction·F PuissantF Leroy
Aug 1, 1987·Gamete Research·P V Dandekar, R H Glass
Oct 1, 1987·Journal of in Vitro Fertilization and Embryo Transfer : IVF·A K PopeJ M Cummins
Aug 1, 1986·Fertility and Sterility·E Snyman, J V Van der Merwe
Mar 1, 1995·Human Reproduction Update·B D Bavister

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Citations

Jan 22, 2013·Reproductive Biology and Endocrinology : RB&E·Waraporn PiromlertamornTeraporn Vutyavanich
Jul 19, 2014·Human Reproduction Update·Elpiniki Chronopoulou, Joyce C Harper
Apr 17, 2012·Gynécologie, obstétrique & fertilité·J-C PontJ-P Wolf
Feb 18, 2010·Reproductive Biomedicine Online·Jose Antonio CastillaLuis Martínez
May 23, 2007·Fertility and Sterility·Eric Gonzalez-MerinoSerena Emiliani

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