Quality control in manufacturing oligo arrays: a combinatorial design approach

Journal of Computational Biology : a Journal of Computational Molecular Cell Biology
R Sengupta, M Tompa

Abstract

The advent of the DNA microarray technology has brought with it the exciting possibility of simultaneously observing the expression levels of all genes in an organism. One such microarray technology, called "oligo arrays," manufactures short single strands of DNA (called probes) onto a glass surface using photolithography. An altered or missed step in such a manufacturing protocol can adversely affect all probes using this failed step and is in general impossible to disentangle from experimental variation when using such a defective array. The idea of designing special quality control probes to detect a failed step was first formulated by Hubbell and Pevzner (1999). We consider an alternative formulation of this problem and use a combinatorial design approach to solve it. Our results improve over prior work in guaranteeing coverage of all protocol steps and in being able to tolerate a greater number of unreliable probe intensities.

References

Oct 23, 1998·Science·S ChuI Herskowitz
Jan 23, 1999·Nature Genetics·R J LipshutzD J Lockhart

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Citations

Dec 21, 2007·Molecular Biotechnology·Larry J Kricka, Stephen R Master
Jun 19, 2001·Current Opinion in Structural Biology·R B Altman, S Raychaudhuri
Aug 3, 2004·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Andrew B KahngAlexander Z Zelikovsky
Apr 14, 2004·Genome Research·Kevin L GundersonMark S Chee
Sep 21, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·V de Mello-Coelho, K L Hess

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