Quality control of laboratory methods for semen evaluation in a multicenter research study

Journal of Andrology
C BrazilStudy for Future Families Research Group

Abstract

Rigorously standardized laboratory protocols and strict quality control (QC) are essential for meaningful comparisons between semen quality data from multiple sites. We describe our experience with the Study for Future Families (SFF), a multicenter study of semen quality in the United States. Detailed protocols were developed, and technicians from each study site attended a training session at the central laboratory. Technicians received blinded replicates from diluted semen specimens for counting by MicroCell and hemacytometer. Sperm motility was assessed using videotaped recordings for simple percent motility and categorical assessment of individual sperm progression as recommended by the World Health Organization (WHO). The mean intertechnician coefficient of variation for individual specimens was 12.6% for MicroCell counts, 15.2% for hemacytometer counts, and 10.5% for percent motility. Intratechnician coefficients of variation averaged 10.3% for MicroCell counts, 12.5% for hemacytometer counts, and 5.2% for percent motility. The average percent differences between the technicians' values and the central standard for individual specimens were 13.5%, 16.6%, and 11.9% for MicroCell counts, hemacytometer counts, and simple per...Continue Reading

References

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Apr 5, 2003·Environmental Health Perspectives·Shanna H SwanUNKNOWN Study For Future Families Research Group
Jun 30, 2004·Journal of Andrology·Charlene BrazilUNKNOWN Study for Future Families Research Group

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Citations

Apr 11, 2008·International Journal of Andrology·Thomas FreourPaul Barriere
Apr 12, 2013·Human Reproduction Update·David MortimerCharles H Muller
Jan 11, 2005·Human Reproduction·Elisabeth CarlsenNiels E Skakkebaek
Feb 10, 2006·International Journal of Andrology·Shanna H Swan
Jun 15, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·T B Haugen
Jan 30, 2010·Asian Journal of Andrology·Charlene Brazil
Nov 26, 2015·Andrology·S W ThurstonS H Swan
Oct 12, 2012·Human Reproduction·M C Sánchez-PozoUNKNOWN Special Interest Group in Andrology of the European Society of Human Reproduction and Embriology
Dec 24, 2013·Human Fertility : Journal of the British Fertility Society·Chey G DearingKevin S Lindsay
Sep 7, 2013·Andrology·J B RedmonUNKNOWN Study for Future Families Research Group
Oct 13, 2004·Journal of Andrology·Lars BjörndahlChristopher L R Barratt
Jun 30, 2004·Journal of Andrology·Charlene BrazilUNKNOWN Study for Future Families Research Group
May 27, 2014·Fertility and Sterility·Teresa JanevicPam Factor-Litvak

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