PMID: 517979May 1, 1979Paper

Quality control of radioimmunoassays for proteins: the first two and a half years of a national scheme for serum growth hormone measurements

Annals of Clinical Biochemistry
W M Hunter, I McKenzie

Abstract

The philosophy and achievements of the first two and a half years of a national quality control scheme for serum growth hormone assays are described. Three serum samples were distributed to participating laboratories every two weeks. A computer-produced summary of the quality control results, which contained scattergrams and a statistical analysis, was returned to participants four weeks after despatch. The performance of 11 experienced (SAS) laboratories was found to fulfil the necessary accuracy criteria, and their results provided the 'reference group mean', which served as the target value. Gross positive bias exhibited by several laboratories was due to the use of assays with inadequate sensitivity, and this practice was eliminated during the first six months. During the course of the Scheme the average bias fell from 30 to 14%. With assays of adequate sensitivity, bias was almost invariably due to the misuse of standards. Median within-laboratory, between-batch precision (CV) improved from 23 to 15%. The best performers achieved between-batch CV of 7% which was 1.5 x their within-batch CV: the worst had three- to four-fold differences between within- and between-batch CV. Rudimentary quality control of interpretation was ...Continue Reading

References

Mar 1, 1970·The Journal of Clinical Endocrinology and Metabolism·S Refetoff, P H Sönksen
Jul 1, 1960·The Journal of Clinical Investigation·R S YALOW, S A BERSON
Aug 24, 1963·Nature·S M GLICKS A BERSON
Mar 28, 1964·British Medical Journal·W M HUNTER, F C GREENWOOD

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Citations

Nov 1, 1980·Clinical Endocrinology·P J HoworthP O'Gorman
Oct 15, 1982·Thrombosis Research·G D LoweC R Prentice
Apr 9, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J A Thomas
Jul 1, 1988·Annals of Clinical Biochemistry·J M Burrin
Sep 1, 1988·Annals of Clinical Biochemistry·R A BacchusT P Whitehead
Nov 1, 1984·Annals of Clinical Biochemistry·P ComptonG Egan

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