Quality control of the traditional Chinese medicine Ruyi jinhuang powder based on high-throughput sequencing and real-time PCR

Scientific Reports
Qiang LiLiran Xu

Abstract

Traditional Chinese medicine (TCM) has been practiced for thousands of years, although concerns about the efficacy, legality, and safety of TCM continue to be raised. Chromatographic studies have detected the presence of heavy metals and plant toxins within some TCM preparations. However, chromatography is not able to identify all of the compounds of TCM, particularly those items that are not clearly labeled on the packaging. The present study aimed to establish a supplemental method that better assesses the ingredient components of TCM preparations.We established an effective approach to screen the biological and toxical composition of TCM based on high-throughput sequencing (HTS), as well as fast detection and validation of the toxical species by real-time PCR, based on ITS2 DNA barcoding. Ruyi jinhuang powder (RHP), a classical herbal prescription containing the toxical herb Arisaematis rhizoma, was chosen to test the method. This method could determine whether the Arisaematis Rhizoma had been replaced by Pinellia pedatisecta in the RHP. The results were validated by real-time PCR. 90% compositions of RHP were identified by ITS2 DNA barcoding, suggesting that more DNA barcoding markers are needed for TCM identification. The ...Continue Reading

References

Jun 8, 2000·The New England Journal of Medicine·J L NortierJ L Vanherweghem
Jul 10, 2003·Trends in Genetics : TIG·Annette W Coleman
Nov 26, 2008·Gene·Alexander KellerMatthias Wolf
Nov 19, 2009·Nucleic Acids Research·Christian KoetschanJörg Schultz
Nov 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·UNKNOWN China Plant BOL GroupGuang-Wen Duan
Dec 13, 2012·Microbial Ecology·Nastassia V PatinMatthew N Ashby
Aug 5, 2014·Biotechnology Advances·Shilin ChenChristine Leon
Jan 8, 2016·Scientific Reports·Jianping HanShilin Chen

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Datasets Mentioned

BETA
SRP130186

Methods Mentioned

BETA
PCR
chip
amplicon sequencing
Illumina sequencing
affects
chips

Software Mentioned

Torrent
BLAST
QuantStudio
QuantStudio 3D
Ion Torrent
Torrent Suite
REF
TCM

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