Quantal release of acetylcholine in mice with reduced levels of the vesicular acetylcholine transporter

Journal of Neurochemistry
Ricardo D F LimaChristopher Kushmerick

Abstract

Mammalian motor nerve terminals contain hundreds of thousands of synaptic vesicles, but only a fraction of these vesicles is immediately available for release, the remainder forming a reserve pool. The supply of vesicles is replenished through endocytosis, and newly formed vesicles are refilled with acetylcholine through a process that depends on the vesicular acetylcholine transporter (VAChT). During expression of short-term plasticity, quantal release can be increased, but it is unknown whether this reflects enhanced recruitment of vesicles from the reserve pool or rapid recycling. We examined spontaneous and evoked release of acetylcholine at endplates from genetically modified VAChT KD(HOM) mice that express approximately 30% of the normal level of VAChT to determine steps rate-limited by synaptic vesicle filling. Quantal content and quantal size were reduced in VAChT KD(HOM) mice compared with wild-type controls. Although high-frequency stimulation did not reduce quantal size further, the post-tetanic increase in end-plate potential amplitude or MEPP frequency was significantly smaller in VAChT KD(HOM) mice. This was the case even when tetanic depression was eliminated using an extracellular solution containing reduced Ca(...Continue Reading

References

Sep 1, 1992·The Journal of Physiology·A I Bain, D M Quastel
Feb 1, 1988·The Journal of Physiology·I Nussinovitch, R Rahamimoff
Jan 1, 1989·Annual Review of Neuroscience·R S Zucker
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B A Bahr, S M Parsons
Jul 1, 1974·The American Journal of Physiology·D F Wilson, L R Skirboll
Feb 1, 1981·The Journal of Physiology·E M McLachlan, A R Martin
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·A RoghaniR H Edwards
Nov 1, 1996·The Journal of Biological Chemistry·H Varoqui, J D Erickson
Apr 1, 1997·Trends in Neurosciences·S A FisherT J Carew
Dec 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S M Parsons
Feb 5, 2002·Annual Review of Physiology·Robert S Zucker, Wade G Regehr
Aug 5, 2003·Neuron·David A RichardsWilliam J Betz
Jun 28, 1954·The Journal of Physiology·J DEL CASTILLO, B KATZ
Jun 26, 2004·Annual Review of Neuroscience·Thomas C Sudhof
Dec 22, 2004·Nature Reviews. Neuroscience·Silvio O Rizzoli, William J Betz
Jul 1, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nathan R WilsonGuosong Liu
Jan 16, 2007·Nature Neuroscience·Teja W Groemer, Jurgen Klingauf
May 18, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lucia TabaresWilliam J Betz
Mar 14, 2008·The European Journal of Neuroscience·Paula P PerissinottiOsvaldo D Uchitel
Feb 24, 2009·Neuroscience and Behavioral Physiology·A L ZefirovG F Sitdikova
May 12, 2009·Nature Neuroscience·Naila Ben Fredj, Juan Burrone
Jul 29, 2009·Molecular and Cellular Biology·Braulio M de CastroVania F Prado
Feb 4, 2010·Molecular and Cellular Biology·Aline LaraSilvia Guatimosim

❮ Previous
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Citations

Dec 27, 2011·Cold Spring Harbor Perspectives in Biology·Randy D Blakely, Robert H Edwards
Oct 24, 2012·Behavioural Brain Research·Xavier De JaegerGrace S Pereira
May 27, 2015·The Journal of Comparative Neurology·Mats Julius StensrudVidar Gundersen
Feb 16, 2013·The Biochemical Journal·Vania F PradoMarco A M Prado
Apr 8, 2014·Neurochemistry International·Ciara A Martin, David E Krantz
Jun 18, 2014·Molecular Immunology·Nadim AkashehRichard W Costello
Jun 18, 2016·Cerebral Cortex·Benjamin KolisnykMarco A M Prado
Dec 21, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·A J da SilvaM A Moret
Dec 15, 2015·Journal of Neurophysiology·Francesca CashRichard A Baines
Mar 13, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Helena JanickovaMarco A M Prado
Sep 25, 2020·ASN Neuro·Daniela F GoncalvesMarco A M Prado
Sep 19, 2019·Muscle & Nerve·Luana Pereira Leite SchetinoCristina Guatimosim
Apr 20, 2020·Biochimica Et Biophysica Acta. Biomembranes·Sonali A DeshpandeDavid E Krantz
Mar 18, 2021·The FEBS Journal·Julliane V Joviano-SantosCristina Guatimosim
Jul 25, 2021·International Journal of Molecular Sciences·Nathalia M PinheiroCarla M Prado
Jul 27, 2021·The European Journal of Neuroscience·Ornela KljakicVania F Prado

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