Quantal release of free radicals during exocytosis of phagosomes

Nature Cell Biology
Anke DiDeborah J Nelson

Abstract

Secretion of lysosomes and related organelles is important for immune system function. High-resolution membrane capacitance techniques were used to track changes in membrane area in single phagocytes during opsonized polystyrene bead uptake and release. Secretagogue stimulation of cells preloaded with beads resulted in immediate vesicle discharge, visualized as step increases in capacitance. The size of the increases were consistent with phagosome size. This hypothesis was confirmed by direct observation of dye release from bead-containing phagosomes after secretagogue stimulation. Capacitance recordings of exocytosis were correlated with quantal free radical release, as determined by amperometry. Thus, phagosomes undergo regulated secretion in macrophages, one function of which may be to deliver sequestered free radicals to the extracellular space.

References

Nov 6, 1992·Science·C E Creutz
Mar 1, 1990·The Journal of General Physiology·G Alvarez de Toledo, J M Fernandez
Aug 1, 1985·The Journal of Clinical Investigation·D J NelsonR C Bone
May 1, 1986·Archives of Biochemistry and Biophysics·B Halliwell, J M Gutteridge
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L J Breckenridge, W Almers
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
May 1, 1996·Journal of Leukocyte Biology·H Tapper
Dec 1, 1996·Journal of Leukocyte Biology·F R DeLeo, M T Quinn
Apr 7, 1997·The Journal of Cell Biology·A RodríguezN W Andrews
Jan 1, 1997·Cell Motility and the Cytoskeleton·E SuzakiS Terakawa
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·A P NarenK L Kirk
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·D J HackamS Grinstein
Jul 31, 1999·The Journal of Biological Chemistry·N J HansenJ M Edwardson
Mar 22, 2000·The Journal of Cell Biology·I MartinezN W Andrews
Apr 4, 2000·Current Biology : CB·J Delon
Apr 14, 2000·Pflügers Archiv : European journal of physiology·K D Gillis
May 3, 2000·The Journal of Cell Biology·L BajnoS Grinstein
Jul 8, 2000·Trends in Cell Biology·N W Andrews
Oct 18, 2000·The Journal of Physiology·M Campos-ToimilP Thomas
Nov 21, 2000·Journal of Neuroscience Methods·F SeguraR Borges
Jan 4, 2001·Current Biology : CB·J Delon, R N Germain
Jun 23, 2001·Biochimica Et Biophysica Acta·T FujiwaraK Akagawa
Aug 2, 2001·The Journal of Biological Chemistry·A DiD J Nelson
Nov 24, 2001·Trends in Neurosciences·S Bajjalieh

❮ Previous
Next ❯

Citations

Jul 13, 2007·Experimental Brain Research·Ning TangJin-Fei Yeo
Apr 25, 2012·Purinergic Signalling·Eduardo R Lazarowski
Oct 22, 2002·Molecular Biology of the Cell·Daniel GotthardtThierry Soldati
Jun 13, 2003·Molecular Biology of the Cell·Anke DiH Clive Palfrey
Mar 14, 2007·Trends in Cell Biology·Maurice B Hallett, Sharon Dewitt
Jul 21, 2004·Current Opinion in Cell Biology·Florence Niedergang, Philippe Chavrier
Nov 8, 2007·Cellular Microbiology·Luisa JordaoElsa Anes
May 21, 2008·Chembiochem : a European Journal of Chemical Biology·Christian AmatoreAlaric C W Koh
Apr 15, 2004·American Journal of Physiology. Renal Physiology·János Peti-PeterdiRobert H Chow
Mar 20, 2003·American Journal of Physiology. Endocrinology and Metabolism·Kazuhiro EtoTakashi Kadowaki
Oct 28, 2019·Traffic·Roni Levin-Konigsberg, Sergio Grinstein
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takato TakenouchiMakoto Hashimoto
Aug 30, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Maha Al-JumaaSharon Dewitt
Jul 16, 2008·Chemical Reviews·Christian AmatoreFrédéric Lemaître

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.