Quantification and characterization of microbial biofilm community attached on the surface of fermentation vessels used in green table olive processing

International Journal of Food Microbiology
Athena GrountaEfstathios Z Panagou

Abstract

The aim of the present study was the quantification of biofilm formed on the surface of plastic vessels used in Spanish-style green olive fermentation and the characterization of the biofilm community by means of molecular fingerprinting. Fermentation vessels previously used in green olive processing were subjected to sampling at three different locations, two on the side and one on the bottom of the vessel. Prior to sampling, two cleaning treatments were applied to the containers, including (a) washing with hot tap water (60 °C) and household detergent (treatment A) and (b) washing with hot tap water, household detergent and bleach (treatment B). Population (expressed as log CFU/cm(2)) of total viable counts (TVC), lactic acid bacteria (LAB) and yeasts were enumerated by standard plating. Bulk cells (whole colonies) from agar plates were isolated for further characterization by PCR-DGGE. Results showed that regardless of the cleaning treatment no significant differences were observed between the different sampling locations in the vessel. The initial microbial population before cleaning ranged between 3.0-4.5 log CFU/cm(2) for LAB and 4.0-4.6 log CFU/cm(2) for yeasts. Cleaning treatments exhibited the highest effect on LAB tha...Continue Reading

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Citations

Nov 28, 2015·International Journal of Food Microbiology·Tânia MateusCélia Quintas
Nov 15, 2015·Applied and Environmental Microbiology·Ángela León-RomeroRufino Jiménez-Díaz
Jan 18, 2019·Annual Review of Food Science and Technology·Avelino Alvarez-OrdóñezPaul D Cotter
Apr 20, 2018·Frontiers in Microbiology·Marco CampusRoberta Comunian
Feb 16, 2020·Foods·Giorgia PerpetuiniAldo Corsetti
Jul 28, 2020·Foods·M Francisca Portilha-CunhaF Xavier Malcata
Sep 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Trond Møretrø, Solveig Langsrud

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