Quantification and Characterization of Phagocytosis in the Soil Amoeba Acanthamoeba castellanii by Flow Cytometry.

Applied and Environmental Microbiology
Simon V AveryD Lloyd

Abstract

Phagocytosis in the common grazing soil amoeba Acanthamoeba castellanii was characterized by flow cytometry. Uptake of fluorescently labelled latex microbeads by cells was quantified by appropriate setting of thresholds on light scatter channels and, subsequently, on fluorescence histograms. Confocal laser scanning microscopy was used to verify the effectiveness of sodium azide as a control for distinguishing between cell surface binding and internalization of beads. It was found that binding of beads at the cell surface was complete within 5 min and 80% of cells had beads associated with them after 10 min. However, the total number of phagocytosed beads continued to rise up to 2 h. The prolonged increase in numbers of beads phagocytosed was due to cell populations containing increasing numbers of beads peaking at increasing time intervals from the onset of phagocytosis. Fine adjustment of thresholds on light scatter channels was used to fractionate cells according to cell volume (cell cycle stage). Phagocytotic activity was approximately threefold higher in the largest (oldest) than in the smallest (newly divided) cells of A. castellanii and showed some evidence of periodicity. At no stage in the cell cycle did phagocytosis ce...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·S C SilversteinZ A Cohn
Dec 1, 1977·Experimental Cell Research·B Bowers
Mar 1, 1991·Reviews of Infectious Diseases·T J ByersE R Hugo
Apr 1, 1989·Journal of Leukocyte Biology·G R Hook, C O Odeyale
Dec 1, 1988·Applied and Environmental Microbiology·C H KingK G Porter
Mar 1, 1987·The Biochemical Journal·D Lloyd
Oct 1, 1971·The Journal of Cell Biology·A G UlsamerE D Korn
Feb 1, 1967·Biochemistry·R A Weisman, E D Korn
Feb 1, 1971·Experimental Cell Research·M Rabinovitch, M J De Stefano
Aug 1, 1983·The Journal of Cell Biology·B Bowers, T E Olszewski
Jan 1, 1981·Annual Review of Microbiology·M Alexander
Sep 1, 1993·The Journal of Eukaryotic Microbiology·T C Hohman, B Bowers
Jun 4, 1993·Journal of Immunological Methods·C P WanB H Lau
May 1, 1988·Applied and Environmental Microbiology·B F SherrF Rassoulzadegan
Mar 5, 1994·Biotechnology and Bioengineering·C HatzisA G Fredrickson
Dec 1, 1981·Microbial Ecology·M Clarholm

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Citations

Feb 12, 2014·Parasitology·Naveed Ahmed Khan, Ruqaiyyah Siddiqui
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Byron F Brehm-Stecher, Eric A Johnson
Feb 25, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Edward A G EllowayStephen N Smith
Jun 19, 2002·Antimicrobial Agents and Chemotherapy·Judith A NobleSidney A Crow
Jul 18, 2006·Nature Reviews. Microbiology·Simon V Avery
May 5, 1999·FEMS Microbiology Letters·J BarkerM W Brown
Sep 5, 2008·Current Protocols in Cytometry·Jonathan Porter
Jun 22, 2012·Protein & Cell·Yan Shi
Mar 20, 2018·Frontiers in Cellular and Infection Microbiology·A Leoni SwartHubert Hilbi
Jun 5, 2003·Microbial Pathogenesis·Naveed Ahmed Khan
Jun 20, 2021·Folia Microbiologica·Mohammad Ridwane MungrooNaveed Ahmed Khan
Mar 28, 2020·ACS Biomaterials Science & Engineering·Michael TimmermannChristine Selhuber-Unkel

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