Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry

Phytochemistry
Márcio Santos SoaresMarcos A Machado

Abstract

A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg mL(-1) respectively, with r(2)>0.999 for all curves. The limits of detection (LOD) for both flavonoids were 0.6 and 0.5 μg mL(-1), respectively, with quantification (LOQ) being 2.0 and 1.0 μg mL(-1), respectively. The quantification method was applied to Citrus sinensis grafted onto C. limonia with and without CVC (citrus variegated chlorosis) symptoms after Xylella fastidiosa infection. The total content of rutin was low and practically constant in all analyses in comparison with hesperidin, which showed a significant increase in its amount in symptomatic leaves. Scanning electron microscopy studies on leaves with CVC symptoms showed vessel occlusion by biofilm, and a crystallized material was noted. Considering the difficulty in isolating these crystals for analysis, tissue sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to confirm the presence of hesperidin at the site of infection. The images constructed from MS/MS data with a specific dia...Continue Reading

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Citations

Feb 24, 2016·Frontiers in Plant Science·Yonghui DongPietro Franceschi
Jun 25, 2016·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Berin A BoughtonUte Roessner
May 9, 2016·Trends in Plant Science·Yonghui DongAsaph Aharoni
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Jul 31, 2019·Journal of Agricultural and Food Chemistry·Shuichi Shimma, Takehito Sagawa

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