Quantification for total demethylation potential of environmental samples utilizing the EGFP reporter gene

Journal of Hazardous Materials
Yan QianXianliang Wang

Abstract

The demethylation potential of pollutants is arguably an innate component of their toxicity in environmental samples. A method was developed for determining the total demethylation potential of food samples (TDQ). The demethylation epigenetic toxicity was determined using the Hep G2 cell line transfected with pEGFP-C3 plasmids containing a methylated promoter of the EGFP reporter gene. The total demethylation potential of the sample extracts (the 5-AZA-CdR demethylation toxic equivalency) can be quantified within one week by using a standard curve of the 5-AZA-CdR demethylation agent. To explore the applicability of TDQ for environmental samples, 17 groundwater samples were collected from heavy polluted Kuihe river and the total demethylation potentials of the sample extracts were measured successfully. Meaningful demethylation toxic equivalencies ranging from 0.00050 to 0.01747μM were found in all groundwater sample extracts. Among 19 kinds of inorganic substance, As and Cd played important roles for individual contribution to the total demethylation epigenetic toxicity. The TDQ assay is reliable and fast for quantifying the DNA demethylation potential of environmental sample extracts, which may improve epigenetic toxicity eva...Continue Reading

References

Jan 10, 2002·Genes & Development·Adrian Bird
Nov 16, 2004·Mutation Research·Eriko Okochi-TakadaToshikazu Ushijima
Mar 2, 2006·Environmental Health Perspectives·Paul A Wade, Trevor K Archer
Mar 3, 2006·Cancer Research·Carlo StresemannFrank Lyko
Nov 17, 2006·Biochemical and Biophysical Research Communications·John F ReichardAlvaro Puga
Feb 7, 2007·Cancer Research·Valentina BollatiAllen S Yang
Jan 13, 2009·American Journal of Respiratory and Critical Care Medicine·Andrea BaccarelliJoel Schwartz
Aug 11, 2009·Current Opinion in Pediatrics·Andrea Baccarelli, Valentina Bollati
Dec 15, 2010·Epigenetics : Official Journal of the DNA Methylation Society·Ivo TenengKenneth S Ramos
May 1, 2009·Environmental Toxicology and Pharmacology·Jiuli ZhangShiwen Xu
Jan 31, 2012·Environmental Science & Technology·Rolf AltenburgerBeate I Escher
Mar 3, 2012·Environmental Health Perspectives·Mohammad Bakhtiar HossainKarin Broberg
Dec 18, 2013·Environmental Science & Technology·Fabien PierronPatrice Gonzalez

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