PMID: 9187379Apr 25, 1997Paper

Quantification of 1,5-anhydro-D-glucitol in urine by automated borate complex anion-exchange chromatography with an immobilized enzyme reactor

Journal of Chromatography. B, Biomedical Sciences and Applications
T TanabeM Yabuuchi

Abstract

HPLC using a borate form of a strongly anion-exchange resin column and an immobilized enzyme reactor for colorimetric detection was used to quantify urinary 1,5-anhydro-D-glucitol. Urine samples were introduced into the system every 7 min without any pretreatment, and after separation of interfering substances in the column, 1,5-anhydro-D-glucitol was successively detected. Quantitative determination of urinary 1,5-anhydro-D-glucitol was possible within the 1.2-300 micromol/l range. The coefficient of variance was less than 3% and the correlation between results obtained with our system (y) and those obtained by gas chromatography-mass spectrometry (x) was y=0.983x-1.287 micromol/l (n=42, r=0.998).

References

Mar 1, 1992·Pflügers Archiv : European journal of physiology·E Pitkänen, O M Pitkänen
Mar 1, 1990·The American Journal of Physiology·T YamanouchiE Miyashita
Jan 15, 1990·Experientia·E Pitkänen, O M Pitkänen
Dec 1, 1987·Journal of Biochemistry·S KametaniH Akanuma
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Sep 1, 1982·Scandinavian Journal of Clinical and Laboratory Investigation·E Pitkänen
Jul 1, 1981·Journal of Biochemistry·H AkanumaY Akanuma

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Citations

Mar 31, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Maria Girelli, Enrico Mattei
Aug 29, 2000·Diabetes Research and Clinical Practice·T KawasakiY Aiso

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