Quantification of 2,4-Diacetylphloroglucinol Produced by Fluorescent Pseudomonas spp. In Vitro and in the Rhizosphere of Wheat.

Applied and Environmental Microbiology
R F BonsallL S Thomashow

Abstract

The broad-spectrum antibiotic 2,4-diacetylphloroglucinol (Phl) is a major determinant in the biological control of a wide range of plant diseases by fluorescent Pseudomonas spp. A protocol was developed to readily isolate and quantify Phl from broth and agar cultures and from the rhizosphere environment of plants. Extraction with ethyl acetate at an acidic pH was suitable for both in vitro and in situ sources of Phl. For soil samples, the addition of an initial extraction step with 80% acetone at an acidic pH was highly effective in eliminating polar organic soil components, such as humic and fulvic acids, which can interfere with Phl detection by high-performance liquid chromotography. The efficiency of Phl recovery from soil by a single extraction averaged 54.6%, and a second extraction added another 6.1%. These yields were substantially greater than those achieved by several standard protocols commonly used to extract polar phenolic compounds from soil. For the first time Phl was isolated from the rhizosphere environment in raw soil. Following application of Pseudomonas fluorescens Q2-87 and the Phl-overproducing strain Q2-87(pPHL5122) to the seeds of wheat, 2.1 and 2.4 (mu)g of Phl/g of root plus rhizosphere soil, respectiv...Continue Reading

References

Oct 1, 1991·Applied and Environmental Microbiology·M N VincentE A Pierson
Mar 1, 1996·Applied and Environmental Microbiology·A J van der BijB Lugtenberg

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Citations

Jul 27, 2001·Applied and Environmental Microbiology·D C GlandorfL C van Loon
Jul 31, 2002·Annual Review of Phytopathology·David M WellerLinda S Thomashow
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Henk-jan SchoonbeekMaarten A De Waard
Apr 7, 2004·Applied and Environmental Microbiology·Monika MaurhoferChristoph Keel
Jun 1, 2004·FEMS Microbiology Ecology·Matthias P LutzBrion Duffy
Feb 1, 2004·FEMS Microbiology Ecology·Anne WindingHap Pritchard
Dec 6, 2011·Applied and Environmental Microbiology·Dmitri V MavrodiDavid M Weller
Jun 14, 2014·Frontiers in Microbiology·Gabriele BergKornelia Smalla
Feb 7, 2003·Applied and Environmental Microbiology·Tommy Harder Nielsen, Jan Sørensen
Jun 12, 2008·Annual Review of Microbiology·Ainslie E F LittleJo Handelsman
Jun 12, 2012·Annual Review of Phytopathology·Jos M Raaijmakers, Mark Mazzola
Sep 20, 2011·Applied and Environmental Microbiology·Marketa Sagova-MareckovaFranz Hadacek

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