PMID: 9436465Jun 1, 1997Paper

Quantification of antigen-specific immunoglobulin A after oral booster immunization with ovalbumin in mice mono-associated with segmented filamentous bacteria or Clostridium innocuum

Immunology Letters
J SnelP J Heidt

Abstract

Segmented filamentous bacteria (SFB) are known to stimulate the mucosal immune system. Here, the effect of SFB on oral booster immunization with ovalbumin was investigated. Mice mono-associated with SFB or Clostridium innocuum were sensitized by intraperitoneal administration of 100 micrograms ovalbumin with Freunds complete adjuvant. After 4 weeks, mice received 80 mg ovalbumin orally. A maximum IgA response was found 5 days after this booster immunization. Comparison of mice with SFB and mice with C. innocuum revealed a much higher level of IgA in the gut lumen and more IgA secreting cells in the lamina propria of the SFB-associated mice. However, no differences between both groups of animals were found in specific levels of IgA secreting cells or luminal IgA against ovalbumin. It is concluded that there is no enhancing effect of SFB after booster immunization when mice are primed intraperitoneally with ovalbumin.

Citations

Jan 10, 2012·Annual Review of Immunology·Kenya Honda, Dan R Littman
Feb 18, 2011·Gut Microbes·Takeshi TanoueKenya Honda
Mar 1, 2012·World Journal of Gastrointestinal Pathophysiology·Linda Chia-Hui YuYen-Hsuan Ni
Mar 6, 2009·Mucosal Immunology·K Honda, K Takeda

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