Quantification of apolipoprotein A-1 in cow serum by single radial immunodiffusion

Journal of Dairy Science
A MazurY Rayssiguier

Abstract

A single radial immunodiffusion assay was developed to determine the concentration of the major apolipoprotein A-I in bovine serum. Assay was performed using agarose gel plates with monospecific rabbit antiserum. Standards made with bovine high density lipoproteins or serum 10 times diluted were applied in 3-microliters amounts to each well in the gel. The assay working range was 2 to 22 mg of apolipoprotein A-I. The intraassay and interassay CV were 3.7 and 4.9%, respectively. Using protein A-I. The intraassay and interassay CY were 3.7 and 4.9%, respectively. Using radial immunodiffusion assay, 23 serum samples from cows at various stages of lactation were analyzed. Apolipoprotein A-I was significantly increased in midlactation compared with concentrations in early lactation or dry period.

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Citations

Dec 1, 1993·Journal of Dairy Science·D Bauchart
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E MarcosY Rayssiguier
Nov 8, 2011·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Lorenza Della DonnaDonald L Puppione

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