Quantification of artemisinin in Artemisia annua extracts by 1H-NMR.

Phytochemical Analysis : PCA
Paula C CastilhoAna I Rodrigues

Abstract

Artemisinin is a polycyclic sesquiterpene lactone that is highly effective against multidrug-resistant strains of Plasmodium falciparum, the etiological agent of the most severe form of malaria. Determination of artemisinin in the source plant, Artemisia annua, is a challenging problem since the compound is present in very low concentrations, is thermolabile and unstable, and lacks chromophoric or fluorophoric groups. The ain of this study was to develop a simple protocol for the quantification of artemisinin in a plant extract using an (1)H-NMR method. Samples were prepared by extraction of leaf material with acetone, treatment with activated charcoal to remove chlorophylls and removal of solvent. (1)H-NMR spectra were measured on samples dissolved in deuterochloroform with tert-butanol as internal standard. Quantification was carried out using the using the delta 5.864 signal of artemisinin and the delta 1.276 signal of tert-butanol. The method was optimised and fully validated against a reference standard of artemisinin. The results were compared with those obtained from the same samples quantified using an HPLC-refractive index (RI) method. The (1)H-NMR method gave a linear response for artemisinin within the range 9.85-97....Continue Reading

References

Jan 1, 1997·Plant Molecular Biology·E Van GeldreE Van den Eeckhout
May 29, 1999·The American Journal of Tropical Medicine and Hygiene·R PriceF Nosten
Feb 9, 2000·Life Sciences·V DhingraM Lakshmi Narasu
Jan 5, 2002·Current Medicinal Chemistry·P Christen, J L Veuthey
Mar 20, 2002·Accounts of Chemical Research·Anne RobertBernard Meunier
Sep 11, 2002·The Lancet Infectious Diseases·Philippe J GuerinNicholas J White
Nov 1, 2002·Journal of Pharmaceutical and Biomedical Analysis·Mojtaba ShamsipurShamsoddin Tabatabaei
Feb 1, 2005·Journal of Natural Products·Guido F PauliDavid C Lankin
Sep 1, 2005·Journal of Agricultural and Food Chemistry·Mingfu WangJames E Simon
Nov 28, 2006·Journal of Natural Products·Alexei A LapkinMalcolm Cutler

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Citations

Sep 19, 2012·Transactions of the Royal Society of Tropical Medicine and Hygiene·Antonella De DonnoFrancesco Paolo Fanizzi
May 6, 2010·Parasitology·E Holmes
Apr 10, 2012·Journal of Natural Products·Guido F PauliDavid C Lankin
Apr 8, 2014·Fitoterapia·Kenny KuchtaHans Wilhelm Rauwald
Aug 21, 2013·Journal of Pharmaceutical and Biomedical Analysis·Patrick GiraudeauSerge Akoka
Sep 11, 2009·Phytochemical Analysis : PCA·Andrea LubbeRobert Verpoorte
Mar 24, 2010·Phytochemical Analysis : PCA·Ning Qing LiuFrank van der Kooy
Apr 5, 2014·Magnetic Resonance in Chemistry : MRC·Patrick Giraudeau
Nov 17, 2019·Magnetic Resonance in Chemistry : MRC·Vassiliki G KontogianniIoannis P Gerothanassis
Aug 21, 2009·Natural Product Reports·Braulio M Fraga
Jul 19, 2020·International Journal of Molecular Sciences·Axelle Septembre-MalaterrePhilippe Gasque
Nov 7, 2008·Phytochemical Analysis : PCA

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