Quantification of attached cells in tissue culture plates and on microcarriers.

Analytical Biochemistry
R Margis, R Borojevic

Abstract

A method for quantification of anchorage-dependent cells in culture on plane surfaces or on microcarriers is proposed. It is based on Coomassie brilliant blue R-250 adsorption, followed by elution of the dye and measurement by spectrophotometry at 595 nm. A linear correlation (r = 0.988 to 0.996) was observed between absorbance and cell number along a large range of cell densities. This technique may be used for monitoring cell growth, from seeding of initial inoculi to scaling up of cultures in bioreactors.

References

Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A López-De León, M Rojkind
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Sep 3, 1985·Journal of Immunological Methods·H Baumgarten

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Citations

Jul 19, 2001·Biotechnology and Bioengineering·P DucommunI W Marison
Mar 6, 2007·Cellular and Molecular Neurobiology·Ariane ZamonerRegina Pessoa-Pureur
Oct 1, 1991·Journal of Biochemical and Biophysical Methods·M C RuizE Cohen
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Oct 20, 2000·Plastic and Reconstructive Surgery·M E DudziakM T Longaker
Apr 6, 2006·Parasitology Research·Alessandra JorqueiraSaulo C Bourguignon
Dec 29, 2004·Biomaterials·V M TatardC N Montero-Menei
Sep 25, 2003·Journal of Cellular Biochemistry·Fabiana M da SilvaFátima C R Guma
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Feb 1, 1994·International Journal of Cancer. Journal International Du Cancer·A MénoretJ Le Pendu
Nov 5, 1999·Analytical Biochemistry·G RaspotnigH J Leis

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