Quantification of Bacterial Twitching Motility in Dense Colonies Using Transmitted Light Microscopy and Computational Image Analysis.

Bio-protocol
Benjamin SmithSuzanne Fleiszig

Abstract

A method was developed to allow the quantification and mapping of relative bacterial twitching motility in dense samples, where tracking of individual bacteria was not feasible. In this approach, movies of bacterial films were acquired using differential interference contrast microscopy (DIC), and bacterial motility was then indirectly quantified by the degree to which the bacteria modulated the intensity of light in the field-of-view over time. This allowed the mapping of areas of relatively high and low motility within a single field-of-view, and comparison of the total distribution of motility between samples.

References

Oct 28, 1999·Microbiology·A B SemmlerJ S Mattick
Jul 27, 2002·Annual Review of Microbiology·John S Mattick
Aug 23, 2003·Infection and Immunity·Irandokht ZolfagharSuzanne M J Fleiszig
Aug 11, 2005·Molecular Microbiology·Lori L Burrows
Jan 13, 2009·Investigative Ophthalmology & Visual Science·Irania AlarconSuzanne M J Fleiszig

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