PMID: 11334343May 4, 2001Paper

Quantification of BNP7787 (dimesna) and its metabolite mesna in human plasma and urine by high-performance liquid chromatography with electrochemical detection

Journal of Chromatography. B, Biomedical Sciences and Applications
Miranda VerschraagenWim J F Van der Vijgh

Abstract

A sensitive and accurate assay was developed and validated to determine BNP7787 (dimesna), a new protector against cisplatin-induced toxicities, and its metabolite mesna in plasma and urine of patients. Both analytes were measured as mesna in deproteinized plasma or in urine diluted with mobile phase using high-performance liquid chromatography with an electrochemical detector provided with a wall-jet gold electrode. The assays for BNP7787 and mesna in deproteinized plasma were linear over the range of 1.6-500 microM and 0.63-320 microM, respectively. In plasma, the mean recovery of BNP7787 over the whole concentration range was 100.6% and of mesna 94.6%. The lower limits of quantitation (LLQs) of BNP7787 and mesna in deproteinized plasma were 1.6 microM and 0.63 microM, respectively. For both compounds the within- and between-day accuracy and precision of the assay was better than 12%. The assays for BNP7787 and mesna in urine were linear over the range of 0.8-1200 microM and 0.63-250 microM, respectively. In urine, the mean recovery of BNP7787 over the whole concentration range was 94.1% and of mesna 93.1%. The LLQ of BNP7787 in urine was 0.8 microM and of mesna 1.6 microM. The within- and between-day accuracy and precision o...Continue Reading

Citations

Aug 29, 2009·Cancer Chemotherapy and Pharmacology·Frederick H HausheerAulma R Parker
May 5, 2010·Cancer Chemotherapy and Pharmacology·F H HausheerH Kochat
May 15, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·E BovenW J F van der Vijgh
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Miranda VerschraagenWim J F van der Vijgh
Jun 12, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Satoshi TakamotoTohru Kojima
Nov 17, 2015·Expert Review of Anticancer Therapy·Dahlia SalmanShereen Nabhani-Gebara
Apr 25, 2003·Journal of Pharmaceutical Sciences·Miranda VerschraagenWim J F Van der Vijgh
Jul 26, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xia ZhengYuan Tian
Apr 24, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Krzysztof KuśmierekEdward Bald
Apr 21, 2011·Journal of Clinical Pharmacology·M J CutlerD J Freeman
Jun 9, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Paulina FurmaniakRafał Głowacki
May 17, 2003·Cancer Chemotherapy and Pharmacology·Miranda VerschraagenWim J F Van der Vijgh
Mar 13, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dakshine ShanmugarajahFrederick H Hausheer

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