Quantification of Botrytis cinerea in Grapevine Bunch Trash by Real-Time PCR

Phytopathology
Melissa Si AmmourVittorio Rossi

Abstract

Quantification of colonization of grape bunch trash by Botrytis cinerea is crucial for Botrytis bunch rot (BBR) control. A previously developed quantitative polymerase chain reaction (qPCR) method was adapted to quantify B. cinerea DNA in grape bunch trash, and a colonization coefficient (CC) was calculated as the ratio between the DNA concentrations of B. cinerea and of Vitis vinifera. CC values increased linearly with the number of conidia of B. cinerea or the quantity of mycelium of B. cinerea added to the bunch trash increased. CC values also increased linearly in bunch trash samples containing increasing percentages of B. cinerea-colonized bunch trash; in the latter samples, CC values were correlated with subsequent assessments of B. cinerea colonization of trash (as determined by plating on agar) and sporulation on the trash (as determined by spore counts after incubation in humid chambers). The qPCR assay was also validated using trash collected from bunches treated or not treated with fungicides in three vineyards in two seasons. CC values reflected the reduction in sporulation and in latent infections of mature berries caused by fungicide application. The qPCR assay enables rapid, specific, sensitive, and reliable quan...Continue Reading

References

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Feb 13, 2001·Letters in Applied Microbiology·S G Edwards, B Seddon
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Citations

Jan 17, 2020·Plant Disease·Giorgia FedeleVittorio Rossi
Aug 28, 2020·Frontiers in Plant Science·Melissa Si AmmourVittorio Rossi

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