Quantification of busulfan in saliva and plasma in haematopoietic stem cell transplantation in children : validation of liquid chromatography tandem mass spectrometry method

Clinical Pharmacokinetics
Manfred RauhW Rascher

Abstract

Busulfan pharmacokinetic studies suggest that an individual dosing strategy may be necessary to optimise systemic exposure in order to decrease toxicity and improve outcome in haematopoietic stem cell transplantation. Therapeutic and toxic effects of the busulfan/cyclophosphamide regimen have been related to the area under the busulfan plasma concentration-time curve. Because of practical limitations in obtaining blood from children, saliva was evaluated as an alternative matrix for therapeutic drug monitoring, offering the advantages of a non-invasive, rapid and easy sampling procedure. Another objective was to evaluate an easy and robust liquid chromatography- tandem mass spectrometry method for plasma and saliva busulfan determination. An online extraction cartridge with column-switching technique, analytical liquid chromatography over a Chromolith RP 18 e column, and tandem mass spectrometry were used to quantify busulfan concentrations in matched plasma and saliva samples. The study population consisted of ten patients, aged 1.3-19 years (median age 11.8 years, seven females, three males), undergoing haematopoietic stem cell transplantation. All patients received busulfan 0.8-1.3 mg/kg orally every 6 hours for a total of 1...Continue Reading

References

Nov 1, 1992·Clinical Pharmacokinetics·R K Drobitch, C K Svensson
Jan 1, 1989·Cancer Chemotherapy and Pharmacology·G VassalJ Lemerle
Apr 1, 1996·Journal of Pharmaceutical Sciences·P B Laub, J M Gallo
Feb 23, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·M H QuerninE Jacqz-Aigrain
Aug 7, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·J E PerisF Torres-Molina
Oct 19, 1999·Medical Oncology·M Hassan
Sep 8, 2000·Clinical Pharmacokinetics·J S McCuneJ T Slattery
May 22, 2001·Therapeutic Drug Monitoring·S N de WildtJ N van Den Anker
Oct 10, 2001·Therapeutic Drug Monitoring·A TabakN Krivoy
Nov 17, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M H QuerninE J Aigrain
Jan 10, 2002·Bone Marrow Transplantation·A M BolingerM J Cowan
Apr 16, 2002·Clinical Therapeutics·Barry D KahanAtholl Johnston
Jun 18, 2003·Cancer Chemotherapy and Pharmacology·Brunhild SchiltmeyerGeorg Hempel
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Sep 23, 2003·Clinical Chemistry·Michael GröschlHelmuth-Günther Dörr
May 29, 2004·Therapeutic Drug Monitoring·Michael TennisonRobert Greenwood
Feb 16, 2005·Anti-cancer Drugs·Doris OechteringJoachim Boos
Sep 22, 2005·Therapeutic Drug Monitoring·M D KelloggN Rifai

❮ Previous
Next ❯

Citations

Nov 19, 2010·Bioanalysis·Dylan M BachWilliam Clarke
Mar 27, 2010·European Journal of Clinical Pharmacology·Roosmarijn F W De CockCatherijne A J Knibbe
Sep 26, 2008·Biomedical Chromatography : BMC·Ramesh MullangiNuggehally R Srinivas
Aug 14, 2013·Biomedical Chromatography : BMC·Kanumuri Siva Rama Raju Wahajuddin
May 16, 2017·Bioanalysis·Michael Gröschl
Jul 27, 2017·Environmental Health Perspectives·Vincent BessonneauStephen M Rappaport
Feb 15, 2018·British Journal of Clinical Pharmacology·Laura HutchinsonBridgeen Callan
Sep 25, 2018·Journal of Clinical Pharmacology·Stephen J Balevic, Michael Cohen-Wolkowiez
May 6, 2011·Therapeutic Drug Monitoring·JoEtta M JuenkeKamisha L Johnson-Davis
May 21, 2020·British Journal of Clinical Pharmacology·Bradley D MenzStephanie E Reuter
May 25, 2011·Journal of Separation Science·Dustin R Bunch, Sihe Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.