Quantification of cell co-migration occurrences during cell aggregation on fibroin substrates

Tissue Engineering. Part C, Methods
Akihisa OtakaNaohide Tomita

Abstract

A quantitative analytical method was proposed for measuring cell co-migration, which was defined as two or more cells migrating together. To accurately identify and quantify this behavior, cell migration on fibroin substrates was analyzed with respect to intercellular distance. Specifically, cell size was characterized by major diameter, and then, based on these measurements and cell center data, a specific threshold distance for defining co-migration was determined after analyzing cell motion using the Voronoi diagram method. The results confirmed that co-migration occurrences of rounded cells were significantly more stable on fibroin than on ProNectin substrates under the present experimental conditions. The cell co-migration analysis method in this article was shown to be successful in evaluating the stability of cell co-migration and also suggested the presence of "critical distance" where two cells interact on fibroin substrates. With further research, the cell co-migration analysis method and "critical distance" may prove to be capable of identifying the aggregation behavior of other cells on different materials, making it a valuable tool that can be used in tissue engineering design.

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Citations

Jul 21, 2015·Journal of Biomedical Materials Research. Part a·Yusuke KambeTsunenori Kameda
Apr 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomoko HashimotoYasushi Tamada

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Software Mentioned

R
MTrackJ
ProNectin
R Foundation for Statistical Computing
ImageJ

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