PMID: 7010857Jan 1, 1981Paper

Quantification of cells in islets of Langerhans using DNA determination

Acta diabetologica latina
J BeckmannU Panten

Abstract

DNA content seems to be an ideal reference parameter for data on secretory function or metabolism of pancreatic islets. The approved fluorometric DNA assay with diaminobenzoic acid (DABA) of Kissane and Robins comprises repeated ethanol extractions of the tissue for removal of lipids from which some DABA-reactive aldehydes may originate. In the present study it is demonstrated that only negligible amounts of DABA-positive material are extractable from islets of Langerhans. Furthermore, it is shown that various substances used in experiments on the endocrine pancreas do not interfere with the DABA-DNA reaction. A modification of the original DABA procedure which does not include ethanol extractions and which is thus more simple and accurate is described for application to pancreatic islets in the absence as well as in the presence of incubation medium. A close linear correlation between islet dry weight and islet DNA content is demonstrated. Islets from rats, normal mice, and ob/ob mice contain 38.3-39.2 ng DNA per microgram dry weight.

References

Aug 15, 1978·Analytical Biochemistry·P S Thomas, M N Farquhar
Mar 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A U Parman
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M A Permutt, D M Kipnis
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jan 1, 1964·Acta Endocrinologica·C HELLERSTROEM

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Citations

Aug 11, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Anna PisaniaClark K Colton
Mar 17, 2010·Critical Reviews in Toxicology·Bertram Price

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