Quantification of cholesterol tracers by gas chromatography--negative ion chemical ionization mass spectrometry

Journal of Mass Spectrometry : JMS
R E OstlundD L Hachey

Abstract

Because of its high sensitivity, gas chromatography negative ion chemical ionization mass spectrometry (GC-NCI-MS) is a potentially valuable analytical tool for the study of cholesterol metabolism. Of several derivatives prepared for potential use in tracer studies pentafluorobenzoyl cholesterol was selected because it formed rapidly at ambient temperature and was stable for long periods, could be detected at a level of 1 fmol, and yielded a mass spectrum in which the molecular ion was the principal component. Hexadeuterated cholesterol tracer ([26,26,26,27,27,27-2H6]cholesterol) could be detected in dilutions up to 2700 in unlabeled cholesterol by selected ion monitoring with a coefficient of variation averaging 3.2%. In seven normal subjects tracer cholesterol was infused intravenously and plasma cholesterol enrichment was determined after 4 h. The measured rapidly miscible cholesterol pool was 391.0 +/- 38.6 mg cholesterol/kg. Negative ion mass spectrometry of pentafluorobenzyol cholesterol will facilitate analysis of both small amounts of natural cholesterol and labeled cholesterol in applications where sensitivity is critical.

Citations

May 3, 2003·Journal of the American Dietetic Association·Curtis A SpilburgRichard E Ostlund
Aug 30, 2005·Isotopes in Environmental and Health Studies·Frans Stellaard
Mar 18, 2006·Isotopes in Environmental and Health Studies·Frans Stellaard, Henk Elzinga
Sep 24, 2011·The American Journal of Clinical Nutrition·Pathmaja ParamsothyRichard E Ostlund
Sep 22, 2012·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Verica Milivojevic, Jonathan Covault
Aug 29, 2006·Journal of Lipid Research·Emily J WestoverRichard E Ostlund
Oct 13, 2001·The Journal of Biological Chemistry·C M CrowderD F Covey
Oct 28, 1999·The American Journal of Clinical Nutrition·R E OstlundW F Stenson
Mar 8, 2002·American Journal of Physiology. Endocrinology and Metabolism·Richard E OstlundCurtis A Spilburg
Jun 7, 2003·The American Journal of Clinical Nutrition·Richard E OstlundWilliam F Stenson
May 31, 2002·The American Journal of Clinical Nutrition·Richard E OstlundWilliam F Stenson
Dec 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·G Beaumier-GallonH Portugal

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