Quantification of colorectal cancer micrometastases in lymph nodes by nested and real-time reverse transcriptase-PCR analysis for carcinoembryonic antigen

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Samuel B HoRobert A Kratzke

Abstract

Reverse-transcriptase PCR (RT-PCR) assays for carcinoembryonic antigen (CEA) have been described to identify lymph node micrometastases. These assays are not quantitative and can be confounded by false-positive results. The purpose of this study was to determine whether quantification of CEA in lymph nodes could more readily identify clinically relevant groups. Specimens included 400 lymph nodes from 64 patients undergoing colon resections. Specimens were tested by immunohistochemistry and by RT-PCR using nested primers for CEA. Specimens from 59 patients that were positive by nested RT-PCR were further quantified by detection of CEA mRNA fluorescence increase at a threshold PCR cycle. CEA was detected by nested RT-PCR analysis in 4 of 34 (12%) nodes of nonneoplastic disease, 2 of 13 (15%) nodes from T(1)N(0) patients, 32 of 81 (40%) nodes of T(2)N(0) patients, 49 of 109 (45%) nodes from T(3)N0 patients, and 92 of 163 (56%) nodes from T(1-4)N(1-2) patients. The overall presence of any RT-PCR-detectable CEA in nodes did not differentiate patient groups. Immunohistochemistry was positive in nodes from 7% of T(3)N(0) patients and 100% of T(1-3)N(1-2) patients. CEA quantification revealed that 0 of 7 patients with nonneoplastic dis...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J ChellyA Kahn
Nov 1, 1987·Archives of Surgery·L Herrera-OrnelasA Mittelman
Apr 21, 1994·The New England Journal of Medicine·C G Moertel
Jul 1, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A ZippeliusK Pantel
Apr 8, 1998·Journal of Surgical Oncology·R CalaluceY W Yesus
Jul 23, 1998·The New England Journal of Medicine·G J LiefersR A Tollenaar
Aug 15, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P J BostickD S Hoon
May 20, 2000·Annals of the New York Academy of Sciences·R O WellerA E Roher
Jun 1, 2000·European Journal of Gastroenterology & Hepatology·G ClarkeP MacMathuna
Sep 15, 2000·Acta Oncologica·P K Lilleng, F Hartveit
Sep 27, 2000·Annals of Surgical Oncology·J TschmelitschA M Cohen
Oct 16, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shingo NouraMorito Monden

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Citations

Nov 23, 2006·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·S GretschelP M Schlag
Jul 2, 2005·Langenbeck's Archives of Surgery·Fabian WolfrumHolger Kalthoff
Sep 11, 2012·Surgery Today·Takashi AkiyoshiToshiaki Watanabe
Dec 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Katherine S GarmanAnil Potti
Nov 10, 2007·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·A SutnarL Holubec
Sep 10, 2005·Expert Review of Molecular Diagnostics·Glen S FrickScott A Waldman
Sep 1, 2007·Expert Opinion on Medical Diagnostics·Jamie Murphy BStephen A Bustin
Aug 4, 2009·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·J G TralhãoF Castro Sousa
Jun 24, 2008·Surgical Oncology·Andreas BembenekPeter M Schlag
Nov 28, 2006·Journal of Biochemical and Biophysical Methods·Chengming WangBernhard Kaltenboeck
Apr 6, 2007·The British Journal of Surgery·T SetoyamaT Aikou
Feb 1, 2006·Molecular Aspects of Medicine·Stephen A Bustin, Reinhold Mueller
Dec 27, 2005·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·K TaniguchiS Uemoto
Nov 23, 2006·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Kiyomi TaniyamaMasato Koseki
Sep 21, 2005·Clinical Science·Stephen A Bustin, Reinhold Mueller
Aug 9, 2019·Applied Immunohistochemistry & Molecular Morphology : AIMM·Olivier DerooSéverine Valmary-Degano
Mar 29, 2014·Expert Review of Anticancer Therapy·Deyong YangLina Wang
Oct 26, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tetsuro SetoyamaTakashi Aikou

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