PMID: 9187377Apr 25, 1997Paper

Quantification of corticosteroids in human plasma by liquid chromatography-thermospray mass spectrometry using stable isotope dilution

Journal of Chromatography. B, Biomedical Sciences and Applications
H ShibasakiY Kasuya

Abstract

Liquid chromatography-thermospray mass spectrometry (LC-TSP-MS) using isotope dilution was investigated for quantitative analysis of cortisol, cortisone, prednisolone and prednisone in human plasma. Complete separation attained by a LiChroCART Supersupher reversed-phase column and elution with 0.05 M ammonium formate-tetrahydrofuran-methanol (180:53:17, v/v/v) resulted in a significantly large isotope effect of the deuterium-labeled analogs on the HPLC behavior and caused difficulty in quantification. Reduction of the isotope effect on the retention times using 0.05 M ammonium formate-acetonitrile (65:35, v/v) permitted accurate quantification of cortisol and cortisone by the isotope dilution LC-TSP-MS, although separation between cortisol and prednisone was incomplete.

References

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Citations

Sep 5, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Atsushi SuzukiTakashi Furuta
Feb 26, 2000·Forensic Science International : Synergy·V CirimeleB Ludes
Dec 14, 2004·Clinical Biochemistry·Jean-Sébastien RaulPascal Kintz
May 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yan ZhangRu-Qin Yu
Sep 4, 2012·Biosensors & Bioelectronics·Masaki YamaguchiVivek Shetty
Mar 2, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Cedric Shackleton
Mar 21, 2012·Psychiatry Research·Jadwiga PiwowarskaJan Pachecka
Aug 12, 2018·Journal of Mass Spectrometry : JMS·Tajudheen K KarattSaraswathy Laya
Jan 5, 2002·Journal of Chromatography. a·K ShimadaT Higashi
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·P Marquet, G Lachâtre
Mar 14, 2007·Analytical Chemistry·Alejandro J RamirezC Kevin Chambliss

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