Quantification of cultivable bacteria and endotoxin in post-treatment apical periodontitis before and after chemo-mechanical preparation.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
M S EndoB P F A Gomes

Abstract

This clinical study was conducted to quantify cultivable bacteria and endotoxin in root canals with post-treatment apical periodontitis by correlating their levels with clinical features and to evaluate the effect of chemo-mechanical preparation (CMP) with 2 % chlorhexidine gel + 17 % EDTA on bacterial and endotoxin removal/elimination. Moreover, target strict Gram-negative anaerobic bacteria were investigated by polymerase chain reaction (PCR). Fifteen teeth with post-treatment apical periodontitis were sampled before (s1) and after (s2) CMP. Culture techniques determined the number of colony-forming units (CFU). PCR (16S rDNA) and limulus amebocyte lysate (LAL) assay were used for bacterial and endotoxin detection, respectively. Prevotella nigrescens (4/15), Prevotella intermedia (2/15), and Tannerella forsythia (2/15) were the most frequently detected species. Endotoxin was recovered in 100 % of the samples. At s1, bacteria and endotoxin were detected at a median value of 5.14 × 10(3) CFU/mL and 3.96 EU/mL, respectively. Higher levels of endotoxin were related to a larger size of radiolucent area (>5 mm) (p < 0.05). CMP was more effective in reducing bacteria (99.61 %) than endotoxin (60.6 %) (both p < 0.05). Our findings in...Continue Reading

References

Jan 1, 1975·Journal of Endodontics·B Schein, H Schilder
Apr 1, 1991·Oral Surgery, Oral Medicine, and Oral Pathology·N HoribaH Nakamura
Dec 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P StashenkoJ D Heeley
Jan 1, 1982·Journal of Endodontics·D L PittsT H Morton
Jun 1, 1982·Scandinavian Journal of Dental Research·L FabriciusA J Möller
Feb 25, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·G SundqvistU Sjögren
May 11, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·H H HancockJ Moiseiwitsch
Aug 4, 2001·Journal of Endodontics·R A BuckH E Hurst
Sep 15, 2001·International Endodontic Journal·V PeciulieneM Haapasalo
Mar 27, 2003·International Endodontic Journal·E T PinheiroF J Souza-Filho
Sep 1, 1965·Oral Surgery, Oral Medicine, and Oral Pathology·S KAKEHASHIR J FITZGERALD
Dec 4, 2003·International Endodontic Journal·J M G TanomaruL A B Silva
May 7, 2004·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Chi-Yuan HongJuo-Song Wang
Dec 3, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P N R Nair
Jul 15, 2005·Journal of Medical Microbiology·Rogerio C JacintoFrancisco J Souza-Filho
Jan 11, 2007·International Endodontic Journal·C Maniglia-FerreiraF J Souza-Filho
Jul 19, 2008·Journal of Endodontics·Isabela N RôçasJosé F Siqueira
Aug 23, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·José F SiqueiraJosé C Provenzano
Jul 2, 2009·Journal of Endodontics·Kumar Subramanian, Andre K Mickel
Jul 16, 2010·Journal of Endodontics·Manoel Eduardo de Lima MachadoCleber Keiti Nabeshima
Jan 18, 2011·Journal of Endodontics·Frederico C MartinhoBrenda P F A Gomes

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Citations

Jul 25, 2013·Journal of Endodontics·Ana Claudia C XavierCláudio A T Carvalho
Jun 14, 2015·Journal of Endodontics·Ericka T PinheiroMárcia P A Mayer
Nov 21, 2014·Clinical Oral Investigations·Frederico C MartinhoIzabel C G Camões
Nov 20, 2013·Archives of Oral Biology·Cássio do NascimentoRubens Ferreira de Albuquerque
Sep 23, 2016·Brazilian Dental Journal·Flávia Goulart da Rosa CardosoMarcia Carneiro Valera
Jun 20, 2013·Brazilian Dental Journal·Brenda P F A GomesCaio C R Ferraz
Sep 22, 2017·European Journal of Dentistry·Frederico Canato MartinhoGustavo Giacomelli Nascimento
Feb 22, 2017·The Open Dentistry Journal·Letizia BorziniLoredana Cerroni
Sep 12, 2019·Brazilian Oral Research·Camila Ambrósio Dias MachadoRogério de Castilho Jacinto
Oct 27, 2018·Brazilian Oral Research·Brenda Paula Figueiredo de Almeida Gomes, Daniel Rodrigo Herrera
Aug 30, 2020·Clinical Oral Investigations·Marlos Barbosa-RibeiroBrenda P F A Gomes

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