Quantification of cyclamate and cyclohexylamine in urine samples using high-performance liquid chromatography with trinitrobenzenesulfonic acid pre-column derivatization

Journal of Chromatography. a
I CasalsL Serra-Majem

Abstract

An HPLC isocratic method with pre-column derivatization and UV detection for the quantification of cyclamate and cyclohexylamine in urine samples is described. The method requires very little sample preparation. Free cyclohexylamine is analysed in a first run and subsequently cyclamate is analysed as cyclohexylamine, after the simple process of oxidation of the sample by means of hydrogen peroxide. Cycloheptylamine is used as internal standard. Trinitrobenzenesulfonic acid (TNBS) appears to be a good reagent for the pre-column derivatization. The time per run is 15 min; the coefficients of variation of the assays range from 1.1 to 5.5%; the limits of detection are 0.09 and 0.11 ppm for cyclohexylamine and cyclamate anion, respectively. The system described has always performed efficiently, with a high degree of stability, in daily routine work.

References

Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M J ScotterN Katz
Apr 17, 2007·Journal of Chromatography. a·Masao HorieKoichi Ito
Jun 9, 2004·The British Journal of Nutrition·Reina García-ClosasCarlos A González
Oct 8, 2008·Public Health Nutrition·Dora RomagueraAntonia Trichopoulou
Jan 17, 2004·Food Additives and Contaminants·L Serra-MajemA G Renwick

Related Concepts

Hydrogen Peroxide
Sugar Substitutes
Cyclamate, Calcium (2: 1) Salt, Dihydrate
Oxidation
Cycloheptylamine
Cyclamate
Spectrophotometry, Ultraviolet
High Pressure Liquid Chromatography Procedure
Trinitrobenzenesulfonic Acid
Cyclohexylamine

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