Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction

Chemical Science
Xiaoyue HanLingxin Chen

Abstract

Cysteine hydropersulfide (Cys-SSH) plays primary roles in the synthesis of sulfur-containing cofactors, regulation of cellular signaling, activation or inactivation of enzyme activities, and modulation of cellular redox milieu. However, its biofunctions need to be further addressed due to the fact that many issues remain to be clarified. Herein, we conceive a novel ratiometric near-infrared fluorescent probe Cy-Dise for the sensitive and selective detection of Cys-SSH in living cells and in vivo for the first time. Cy-Dise is composed of three moieties: bis(2-hydroxyethyl) diselenide, heptamethine cyanine, and d-galactose. Cy-Dise exhibits a satisfactory linear ratio response to Cys-SSH via a selenium-sulfur exchange reaction in the range of 0-12 μM Cys-SSH. The experimental detection limit is determined to be 0.12 μM. The results of ratio imaging analyses confirm the qualitative and quantitative detection capabilities of Cy-Dise in HepG2 cells, HL-7702 cells, and primary hepatocytes. The level changes of Cys-SSH in cells stimulated by some related reagents are also observed. The probe is also suitable for deep tissue ratio imaging. Organ targeting tests with Cy-Dise in normal Spraque-Dawley (SD) rats and Walker-256 tumor SD ra...Continue Reading

References

Aug 1, 1986·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J I Toohey
Feb 19, 1998·Archives of Biochemistry and Biophysics·L BaoJ P Kraus
Aug 5, 1997·Chemical Reviews·A. Prasanna de SilvaTerence E. Rice
Dec 25, 2002·Free Radical Biology & Medicine·Carmela Loguercio, Alessandro Federico
Apr 1, 1960·Archives of Biochemistry and Biophysics·D CAVALLINIB MONDOVI
Jun 8, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dean P JonesWard G Kirlin
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·John D HayesIan R Jowsey
Mar 21, 2006·Nature Chemical Biology·Eugene G Mueller
May 27, 2010·Chemical Society Reviews·Xiaoqiang ChenJuyoung Yoon
Sep 3, 2010·The Journal of Organic Chemistry·Daniel SteinmannWillem H Koppenol
Sep 11, 2010·Chemical Communications : Chem Comm·Animesh SamantaYoung-Tae Chang
Feb 10, 2011·Analytical Biochemistry·John I Toohey
Feb 15, 2011·Biochimica Et Biophysica Acta·Sangita Singh, Ruma Banerjee
Jul 30, 2011·Nature Methods·Tasuku Ueno, Tetsuo Nagano
Dec 17, 2011·Journal of the American Chemical Society·Min Hee LeeJong Seung Kim
Jun 19, 2012·Chemical Reviews·Yuming YangFuyou Li
Jul 12, 2012·Nature Reviews. Molecular Cell Biology·Bindu D Paul, Solomon H Snyder
Mar 16, 2013·British Journal of Pharmacology·Antonia AsimakopoulouAndreas Papapetropoulos
Mar 22, 2013·Chemical Reviews·Candice E Paulsen, Kate S Carroll
Apr 6, 2013·ACS Chemical Biology·Jia Pan, Kate S Carroll
May 22, 2013·Chemical Society Reviews·Hyo Sung JungJuyoung Yoon
May 25, 2013·Chemical Society Reviews·Caixia YinSidian Li
Jul 3, 2013·Methods : a Companion to Methods in Enzymology·Changyuan LuSteven S Gross
Nov 30, 2013·Angewandte Chemie·Dehui ZhangMing Xian
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wen ChyanRobert J Radford
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tomoaki IdaTakaaki Akaike
May 9, 2014·Journal of the American Chemical Society·Chunrong LiuMing Xian
Jul 18, 2014·Chemical Communications : Chem Comm·Fabiao YuLingxin Chen

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Citations

Mar 3, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuhui MenJianhong Wang
Nov 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Liming ZhaoZhipeng Liu
Mar 21, 2017·Chemical Society Reviews·Min GaoLingxin Chen
Feb 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Longbin XuXinghua Wang
Nov 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Min GaoLingxin Chen
May 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Min GaoLingxin Chen
Feb 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ariq AbdillahDavid G Churchill
Feb 16, 2021·Analytica Chimica Acta·Gauri S MalankarSudesh T Manjare
Nov 8, 2017·Chemical Reviews·Milos R FilipovicRuma Banerjee
Apr 19, 2017·Journal of the American Chemical Society·Jean-Philippe R ChauvinDerek A Pratt
Nov 19, 2021·Analytical Chemistry·Amit Kumar SinghN D Pradeep Singh

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Methods Mentioned

BETA
fluorescence imaging
confocal microscopy
flow cytometry
xenografts

Software Mentioned

Image
Dise
Pro Plus
Cy

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