Quantification of Dehalospirillum multivorans in Mixed-Culture Biofilms with an Enzyme-Linked Immunosorbent Assay.

Applied and Environmental Microbiology
P Bauer-KreiselH Scholz-Muramatsu

Abstract

A fast, highly selective and sensitive method to quantify specific biomasses in mixed-culture biofilms is described. It consists of detachment of a biofilm from its support material, resolution of the detached biofilm flocs in order to separate the enclosed cells and antigens, and quantification of specific biomass by an enzyme-linked immunosorbent assay.

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Citations

Jun 1, 1997·Current Opinion in Biotechnology·A J Stams, S J Oude Elferink
Dec 19, 2000·Enzyme and Microbial Technology·H Smidt de Vos WM

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