Apr 27, 2018

Quantification of DNA methylation and hydroxymethylation in Alzheimer's disease mouse model using LC-MS/MS

Journal of Mass Spectrometry : JMS
Qionglin HuangChun Cai

Abstract

Ambiguous alteration patterns of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) involved in Alzheimer's disease (AD) obstructed the mechanism investigation of this neurological disorder from epigenetic view. Here, we applied a fully quantitative and validated LC-MS/MS method to determine genomic 5mC and 5hmC in the brain cortex of 3 month-aged (12, 15, and 18 month) AD model mouse and found significant increases of 5mC and 5hmC levels in different months of AD mouse when compared with age-matched wild-type control and exhibited rising trend from 12-month to 18-month AD mouse, thereby supporting genomic DNA methylation and hydroxymethylation were positively correlated with developing AD.

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Mentioned in this Paper

Familial Alzheimer Disease (FAD)
DNA Methylation [PE]
Cortex Bone Disorders
Adrenal Cortex Diseases
Genome
Brain
DNA Methylation
Alzheimer's Disease
Tandem Mass Spectrometry
Study of Epigenetics

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