Quantification of Double Stranded DNA Breaks and Telomere Length as Proxies for Corneal Damage and Replicative Stress in Human Keratoconus Corneas

Translational Vision Science & Technology
Robert P L WisseJasper CA Broen

Abstract

The pathogenesis of keratoconus (KC) is multifactorial, and associated with oxidative stress and subsequent DNA damage. We investigate differences in DNA damage and replicative stress in patients with KC, and in healthy and diseased controls. We obtained 64 corneal buttons from 27 patients with KC after corneal transplant surgery, 21 with a decompensated graft (DG), and 16 healthy controls (HC). The amount of intact Alu elements per genome copy as measured by quantitative polymerase chain reaction (qPCR) was used to quantify intact DNA. Telomere length was measured as a proxy for replicative stress. In addition, telomerase reverse transcriptase (hTERT) gene expression level was assessed. Mean (± standard deviation [SD]) DNA damage was similar between the KC (5.56 ± 14.08), DG (3.16 ± 8.22), and HC (3.51 ± 6.66) groups (P = 0.807). No associations were found between DNA damage and patient age (P = 0.523), atopic constitution (P = 0.240), or contact lens wear (P = 0.393). Telomere length differed (P = 0.034), most notably in the KC group, and hTERT was not detected in any corneal sample. Three cross-linked (CXL) KC corneas did not contain significantly more DNA damage (×2.6, P = 0.750). Based on these findings, differences in act...Continue Reading

References

Dec 31, 1997·Mutation Research·E W Englander, B H Howard
Mar 11, 1998·Survey of Ophthalmology·Y S Rabinowitz
Jul 25, 2000·The British Journal of Ophthalmology·A M BawazeerB Lorimer
Feb 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rajeev BuddiDonald J Brown
Jul 17, 2004·Cornea·Batool JafriR Doyle Stulting
Mar 26, 2005·Investigative Ophthalmology & Visual Science·Shari R AtilanoM Cristina Kenney
Apr 18, 2006·Current Opinion in Cell Biology·Ignacio FloresMaria A Blasco
May 15, 2008·BioTechniques·Nathan O'CallaghanMichael Fenech
May 5, 2009·Cornea·Shari R AtilanoM Cristina Kenney
Oct 23, 2010·Methods in Molecular Biology·Maurizio Cardelli
Sep 26, 2013·International Journal of Molecular Sciences·Katarzyna A WojcikJacek P Szaflik
Feb 22, 2014·Investigative Ophthalmology & Visual Science·Ronald H SilvermanDan Z Reinstein
Apr 10, 2014·Scientific Reports·D KaramichosJ D Zieske
Dec 30, 2014·Journal of Cataract and Refractive Surgery·Marco LombardoGiuseppe Lombardo
Mar 12, 2015·PLoS Genetics·Maria E MoralesPrescott L Deininger
Apr 29, 2015·Progress in Retinal and Eye Research·Nick Di Girolamo
Jun 17, 2015·Survey of Ophthalmology·Jack S ParkerGerrit R J Melles
Aug 4, 2015·The Ocular Surface·Robert P L WisseAllegonda Van der Lelij

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Citations

May 15, 2021·Investigative Ophthalmology & Visual Science·James W FosterUri S Soiberman
Sep 4, 2021·Translational Vision Science & Technology·Dorota M Nowak-MalczewskaMarzena Gajecka

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