Quantification of Endogenous Cholesterol in Human Serum on Paper Using Direct Analysis in Real Time Mass Spectrometry

Analytical Chemistry
Hua-Yi HsiehCheng-Chih Hsu

Abstract

Blood testing for endogenous small metabolites to determine physiological and biochemical states is routine for laboratory analysis. Here we demonstrate that by combining the commercial direct analysis in real time (DART) ion source with an ion trap mass spectrometer, native cholesterol in its free alcohol form is readily detected from a few hundred nanoliters of human serum loaded onto chromatography paper. Deuterium-labeled cholesterol was used as the internal standard to obtain the absolute quantity of the endogenous cholesterol. The amount of the cholesterol measured by this paper-loaded DART mass spectrometry (pDART-MS) is statistically comparable with that obtained by using commercially available fluorometric-enzymatic assay and liquid chromatography/mass spectrometry. Furthermore, sera from 21 participants at three different time points in an ultramarathon were collected to obtain their cholesterol levels. The test requires only very minimal sample preparation, and the concentrations of cholesterol in each sample were acquired within a minute.

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Citations

Dec 7, 2018·The Analyst·Fan PuZheng Ouyang
Apr 17, 2019·Journal of Food and Drug Analysis·Li-Hua LiCheng-Chih Hsu
Apr 22, 2021·Lab on a Chip·Wen-Chin LeeLung-Ming Fu
Jun 16, 2021·Mass Spectrometry Reviews·Hanlu YueYixiang Duan
Jun 13, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Valentin SchoopDoris Höglinger
May 13, 2021·Annual Review of Analytical Chemistry·Suji LeeAbraham K Badu-Tawiah
Oct 14, 2017·Analytical Chemistry·Max HechtKarin Kipper
Feb 22, 2018·Analytical Chemistry·Huiying MaDavid Da Yong Chen
Nov 6, 2020·Analytical Chemistry·Cheng-Yu WengCheng-Chih Hsu
Aug 24, 2021·Analytical and Bioanalytical Chemistry·Sophie Bravo-Veyrat, Gérard Hopfgartner
Nov 2, 2019·Analytical Chemistry·Devin J SwinerAbraham K Badu-Tawiah

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