Quantification of Epstein-Barr Virus and Human Cytomegalovirus in Chronic Periodontal Patients

Jundishapur Journal of Microbiology
Hengameh KhosropanahMarzieh Jamalidoust

Abstract

Although studies focused mainly on the identification of periopathogenic bacteria, recent reports have suggested that various herpes viruses may also be involved in the occurrence and progression of different forms of periodontal diseases. This study aimed to compare the prevalence and load of Epstein-Barr Virus (EBV) and Human Cytomegalovirus (HCMV) in subgingival tissue specimens between chronic periodontitis and healthy sites. A total of 60 samples from the systematically healthy patients with chronic periodontitis participated in this study (mean age, 35 ± 7). Clinical periodontal evaluation included the plaque index (PI) (Loe and Silness), bleeding on probing (BOP) (O'Leary), bleeding index, periodontal pocket depth (PPD) and clinical attachment level measurement. Tissue specimens harvested from > 6 mm periodontal pockets and from ≤ 3 mm sulcus depth in a quadrant of the same patient using periodontal curettes. Moreover, the unstimulated whole saliva was gathered as a shedding medium. A Taq-man Real-Time Polymerase Chain Reaction assay was used to identify genomic copies of periodontal HCMV and EBV. Data were analyzed by the Wilcoxon-signed ranks and Friedman tests using the SPSS 16 software. Out of 60 samples of subgingiv...Continue Reading

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Citations

Aug 2, 2017·Periodontology 2000·Jørgen Slots
Mar 7, 2021·Journal of Clinical Medicine·Maria ContaldoRosario Serpico
Sep 1, 2021·Periodontology 2000·April MartínezYvonne L Kapila
Mar 14, 2020·Microbial Pathogenesis·Charles V OlivieriSéverine Vincent-Bugnas

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Methods Mentioned

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